论文部分内容阅读
目的:探讨抑癌基因p16和凋亡抑制基因bcl-2在子宫内膜增生过长、子宫内膜癌中的表达及其意义。方法:采用免疫组织化学方法检测并比较66例子宫内膜增生过长、子宫内膜癌组织中p16、bcl-2的表达。结果:从简单型、复杂型、复杂型伴非典型增生过长到子宫内膜癌p16表达率依次下降,分别为78.6%、57.1%、66.6%和40.5%,子宫内膜癌p16阳性表达率明显低于子宫内膜增生过长(P<0.05),子宫内膜癌中不同组织学分型及分级p16阳性表达差异无统计学意义(P>0.05)。bcl-2在子宫内膜癌与复杂型、非典型增生过长中的表达差异无统计学意义(P>0.05),但简单型增生过长与复杂型、非典型增生过长和子宫内膜癌中的表达差异有统计学意义(P<0.05)。bcl-2表达随着分化程度降低而降低(P<0.05)。结论:p16、bcl-2在子宫内膜癌的发生中起着一定的作用,尤其是bcl-2在复杂型、非典型增生过长中高表达,可作为临床诊断的辅助指标。
Objective: To investigate the expression of p16 and bcl-2 in endometrial hyperplasia and endometrial carcinoma and its significance. Methods: Immunohistochemistry was used to detect and compare the expression of p16 and bcl-2 in 66 cases of endometrial hyperplasia and endometrial carcinoma. Results: The rate of p16 expression from simple, complicated and complicated with atypical hyperplasia to endometrial carcinoma decreased successively, which were 78.6%, 57.1%, 66.6% and 40.5%, respectively. The positive rate of p16 in endometrial carcinoma (P <0.05). There was no significant difference between different histological types and grade p16 expression in endometrial carcinoma (P> 0.05). There was no significant difference in the expression of bcl-2 between endometrial carcinoma and complicated and atypical hyperplasia (P> 0.05), but the simple hyperplasia and complex type, the atypical hyperplasia and the endometrium The difference was statistically significant (P <0.05). The expression of bcl-2 decreased with the degree of differentiation decreasing (P <0.05). Conclusion: p16 and bcl-2 play a role in the pathogenesis of endometrial carcinoma. In particular, bcl-2 is overexpressed in complex and atypical hyperplasia, which may serve as an auxiliary index for clinical diagnosis.