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目的探讨接受助孕治疗的不孕症夫妇支原体和衣原体携带情况及其与体外受精/卵细胞质内单精子注射结局的关系。方法选择2009年3月至2011年9月北京大学第一医院生殖与遗传医疗中心无下生殖道感染症状且接受助孕治疗的159对不孕症夫妇,在取卵日或人工授精日分别取宫颈分泌物和精液,采用聚合酶链反应(PCR)法进行支原体和衣原体的检测。结果女性患者支原体阳性29例(18.2%,29/159),其中解脲支原体(ureaplasma urealyticum,UU)和人型支原体(mycoplasma hominis,Mh)分别为26例(16.4%,26/159)和3例(1.9%,3/159),未检出衣原体。男性患者支原体阳性4例(2.5%,4/159),均为UU;衣原体阳性3例(1.9%,3/159)。输卵管性不孕症患者支原体阳性10例(16.9%,10/59),非输卵管性不孕症患者支原体阳性19例(19.0%,19/100),两者比较,差异无统计学意义(P>0.05)。女性支原体阴性组和阳性组的获卵数[(11.8±6.0)个,(14.6±6.2)个]、优质胚胎率(37.6%,43.2%)和临床妊娠率(48.2%,30.0%)比较,差异无统计学意义(P>0.05)。结论进入助孕治疗周期的不孕症夫妇仍有一定的生殖道支原体和衣原体携带率,支原体携带与临床妊娠结局无明显关系。
Objective To investigate the relationship between carriage of Mycoplasma and Chlamydia in infertile couple and its relationship with in vitro fertilization / single sperm injection in oocyte. Methods From March 2009 to September 2011, Peking University First Hospital, Peking University First Hospital reproductive and genetic medical center without symptoms of genital tract infection and received 159 cases of pregnancy-assisted treatment couples infertility, ovum retrieval or artificial insemination days were taken Cervical secretions and semen, the use of polymerase chain reaction (PCR) for mycoplasma and chlamydia detection. Results The positive rate of mycoplasma in female patients was 29 (18.2%, 29/159). The ureaplasma urealyticum (UU) and mycoplasma hominis (Mh) were 26 (16.4%, 26/159) Cases (1.9%, 3/159), no detection of chlamydia. Mycoplasma positive in 4 male patients (2.5%, 4/159) were UU; 3 were positive for Chlamydia (1.9%, 3/159). Tubal infertility in patients with mycoplasma positive in 10 cases (16.9%, 10/59), non-tubal infertility patients with mycoplasma in 19 cases (19.0%, 19/100), the two compared, the difference was not statistically significant (P > 0.05). (11.8 ± 6.0), (14.6 ± 6.2)], high quality embryos (37.6%, 43.2%) and clinical pregnancy rates (48.2%, 30.0%) in the negative and positive groups of mycoplasmas. The difference was not statistically significant (P> 0.05). Conclusion There is still a certain prevalence of mycoplasma and chlamydia in infertile couples with cycles of pregnancy-assisted treatment. Mycoplasma-carrying is not related to clinical pregnancy outcome.