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目的 研究雷帕霉素 (RPM)对人树突状细胞 (DC)功能的影响。方法 分离外周血单个核细胞 (PBMC) ,在含粒细胞巨噬细胞集落刺激因子 (GM CSF)、白介素 4 (IL 4 )以及有或无RPM的培养条件下制备DC。用流式细胞仪检测DC表面共刺激分子CD86 (B7 2 )的表达 ;混合淋巴细胞反应(MLR)检测DC对同种异体T淋巴细胞的刺激能力 ;ELISA法测定MLR上清液中的细胞因子。结果 与对照组比较 ,经RPM处理的DC表面CD86的表达明显降低 ;对T淋巴细胞刺激的能力下降 ;MLR中致炎细胞因子 (IL 1β ,IL 6 ,TNF a)浓度降低。RPM的这些作用呈浓度依赖性。 结论 雷帕霉素对人树突状细胞功能有明显的抑制作用 ,可能是其防止支架术后再狭窄的机制之一
Objective To investigate the effect of rapamycin (RPM) on the function of human dendritic cells (DCs). Methods Peripheral blood mononuclear cells (PBMCs) were isolated and cultured in the presence of GM CSF, IL - 4 and with or without RPM. The expression of costimulatory molecule CD86 (B7 2) on DCs was detected by flow cytometry. The ability of DC to stimulate allogeneic T lymphocytes was measured by mixed lymphocyte reaction (MLR). The cytokines . Results Compared with the control group, the expression of CD86 on the surface of RPM-treated DCs was significantly decreased; the ability to stimulate T lymphocytes was decreased; and the concentrations of pro-inflammatory cytokines (IL 1β, IL 6 and TNFα) in MLR were decreased. These effects of RPM are concentration-dependent. Conclusion Rapamycin can significantly inhibit the function of human dendritic cells and may be one of the mechanisms to prevent the restenosis after stenting