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研究湖南产水菖蒲化学成分及其体外抑菌活性。利用多种色谱技术分离水菖蒲化学成分,结合理化和波谱数据鉴定化合物的结构,并采用96孔板法测定化合物的体外抑菌活性。水菖蒲中分离得到11个倍半萜类化合物,经波谱分析鉴定为4β,6β-dihydroxy~(-1)α,5β(H)-guai-9-ene(1),4β,6β-dihydroxy~(-1)α,5β(H)-guai~(-1)0(14)-ene(2),teuclatriol(3),异菖蒲二醇(4),菖蒲二醇(5),calamusin H(6),oxyphyllenodiols A(7),oplodiol(8),菠萝香藤素(9),表水菖蒲酮(10)和bullatantriol(11),其中,化合物9为首次从菖蒲属植物中分离得到。体外抑菌实验表明,化合物3,7~9和11具有显著地抑菌活性,化合物7对金黄色葡萄球菌(MBC 12.50 mg·L~(-1))、化合物9对4种供试菌(MBC 20.00 mg·L~(-1))、化合物11对绿脓杆菌(MBC 12.50mg·L~(-1))均具有良好的杀菌作用。
To study the chemical constituents of Acorus calamus in Hunan and its antibacterial activity in vitro. The chemical constituents of Acorus calamus were isolated by a variety of chromatographic techniques. The structures of compounds were identified by physico-chemical and spectral data. The antibacterial activities of the compounds in vitro were determined by 96-well plate assay. Eleven sesquiterpenoids were isolated from Acorus calamus and identified as 4β, 6β-dihydroxy ~ (-1) α, 5β (H) -guai-9-ene (-1) α5β (H) -guai ~ (-1) 0 (14) -ene (2), teuclatriol (3), calamusin H 6), oxyphyllenodiols A (7), opal (8), pineapple (9), calafate (10) and bullatantriol (11). Among them, compound 9 was isolated from Acorus for the first time. In vitro antibacterial experiments showed that compounds 3, 7-9 and 11 had significant antibacterial activities against compounds of Staphylococcus aureus (MBC 12.50 mg · L -1) and compounds 9 MBC 20.00 mg · L -1, Compound 11 had good bactericidal activity against Pseudomonas aeruginosa (MBC 12.50 mg · L -1).