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本文采用ＮＡＤＰＨｄ组织化学、图象分析及听觉电生理技术，研究豚鼠衰老过程中血管纹一氧化氮合酶（ＮＯＳ）活性的变化与蜗内电位（ＥＰ）及复合动作电位（ＣＡＰ）的关系，探讨ＮＯＳ在老年性聋发病过程中的可能作用。结果发现，３月龄豚鼠ＥＰ值最大，为８６３２±３２４ｍＶ，随着豚鼠的衰老ＥＰ值逐渐下降，ＣＡＰＮ１波振幅的变化规律与ＥＰ的变化规律一致。ＣＡＰＮ１波潜伏期有逐渐延长的趋势。组织化学染色表明，ＮＯＳ阳性反应主要位于血管纹边缘细胞的胞浆内，随着豚鼠的衰老，血管纹ＮＯＳ阳性反应逐渐减弱，除２周龄组外，ＮＯＳ活性与ＥＰ及ＣＡＰＮ１波振幅有正相关趋势。结果提示，耳蜗血管纹ＮＯＳ可能参与内耳微循环及微环境稳定的调节，血管纹ＮＯＳ含量或活性的降低可能是老年性聋发病的重要因素
In this paper, NADPH d histochemistry, image analysis and auditory electrophysiological techniques to study the changes of vascular smooth muscle nitric oxide synthase (NOS) activity in guinea pigs during aging and intracochlear potential (EP) and compound action potential (CAP) To explore the possible role of NOS in the pathogenesis of senile deafness. The results showed that the EP value of guinea pigs at 3 months was the highest (8632 ± 324mV). As the EP value of guinea pigs decreased gradually, the amplitude of CAPN1 wave was consistent with that of EP. CAPN1 wave latency has gradually extended the trend. Histochemical staining showed that the positive reaction of NOS mainly located in the cytoplasm of marginal cells of the stria vascularis. With the aging of guinea pigs, the positive reaction of NOS in the stria vascularisation gradually weakened. Except for the 2-week-old group, the amplitude of NOS activity was positively correlated with the amplitude of EP and CAPN1 Related trends. The results suggest that the NOS of the stria vascularis may be involved in the regulation of the inner ear microcirculation and microenvironment, and the reduction of the NOS content or activity of the vascular pattern may be an important factor in the development of senile deafness