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目的观察干扰素α-2b联合5-氟尿嘧啶(5-FU)对肝癌细胞株HepG2在体外的杀伤抑制作用,同时检测药物作用后肝癌细胞突变型P53的表达情况。方法用不同浓度的干扰素α-2b(100、500、1000、2000 U/ml)和5-FU(10μg/ml)联合对肝癌细胞株作用不同时间(24、48、72 h),用单四唑(MTT)法检测细胞生长抑制率,进而运用PV-9000二步法对突变型P53表达情况进行测定。结果干扰素α-2b和5-FU联合效果明显优于单用,并与对照组差异有统计学意义(P<0.05)。经药物处理后,突变型P53表达差异有统计学意义(P<0.05)。结论干扰素α-2b和5-氟尿嘧啶能有效抑制肝癌细胞的生长,导致突变型P53因子表达的下调而发挥作用。
Objective To observe the inhibitory effect of interferon α-2b combined with 5-fluorouracil (5-FU) on HepG2 cells in vitro and to detect the expression of mutant P53 in hepatoma cells after drug treatment. Methods HepG2 cells were treated with interferon α-2b (100, 500, 1000 and 2000 U / ml) and 5-FU (10 μg / ml) for different times (24, 48 and 72 h) Tetrazole (MTT) method was used to detect cell growth inhibition rate, and then the PV-9000 two-step method was used to determine the expression of mutant P53. Results The combination effect of interferon α-2b and 5-FU was better than that of the control alone (P <0.05). After drug treatment, the expression of mutant P53 was significantly different (P <0.05). Conclusion Interferon α-2b and 5-fluorouracil can effectively inhibit the growth of hepatocellular carcinoma cells and lead to the down-regulation of the expression of mutant P53 gene.