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【目的】研究来自我国不同地区的45株无柄灵芝菌株的遗传多样性。【方法】利用ITS、TEF1-α和LSU多基因分析及SRAP分子标记两种方法,对供试无柄灵芝菌株进行聚类分析和遗传多样性研究。【结果】筛选出8对SRAP引物共扩增出95条条带,其中具有多态性条带79条,平均多态性比例为82.4%,多态性信息含量(PIC)变幅在0.28-0.43,平均为0.38。ITS、TEF1-α和LSU多基因序列分析结果显示,同一地域的部分菌株聚在一起,亲缘关系较近,而地域相隔较远的部分菌株也聚在同一个进化支上,其亲缘关系也很近,这与SRAP聚类分析结果相吻合。【结论】无柄灵芝菌株遗传多样性较为丰富,其遗传相似性与地理分布存在一定的相关性,ITS、TEF1-α、LSU基因及多基因分析更适合无柄灵芝分类鉴定,而SRAP分子标记更适合于无柄灵芝遗传多样性分析。
【Objective】 The objective of this study was to investigate the genetic diversity of 45 untreated Ganoderma lucidum strains from different regions in China. 【Method】 Cluster analysis and genetic diversity of the tested Ganoderma lucidum were carried out using the methods of ITS, TEF1-α and LSU and SRAP molecular markers. 【Result】 Nine pairs of SRAP primers were screened out, of which 79 bands were polymorphic. The average polymorphism percentage was 82.4%. The polymorphism information content (PIC) ranged from 0.28- 0.43, averaging 0.38. The results of ITS, TEF1-α and LSU multi-gene sequence analysis showed that some strains in the same region clustered together and the genetic relationship was close, while some strains far apart also clustered in the same evolutionary branch. Nearly, this is consistent with the results of SRAP clustering analysis. 【Conclusion】 The genetic diversity of stemless Ganoderma lucidum is more abundant, and its genetic similarity is related to its geographical distribution. ITS, TEF1-α, LSU gene and polygene analysis are more suitable for the classification and identification of Atriplex lucidum, while SRAP molecular marker More suitable for the analysis of stemless Ganoderma lucidum genetic diversity.