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为研究FTY720诱导K562细胞凋亡与线粒体途径的关系。体外培养人白血病K562细胞,用不同浓度FTY720处理细胞24 h,台盼蓝染色法检测K562细胞存活率;流式细胞术分析细胞凋亡和线粒体功能。免疫印迹技术检测线粒体相关蛋白Bcl-2和Bax的表达。收集FTY720处理48 h细胞,流式细胞技术检测细胞周期。结果表明:随FTY720浓度的增加,K562细胞的存活率下降,细胞凋亡增加,线粒体膜电位下降,促凋亡蛋白Bax表达明显增加。细胞周期分析发现,Sub G1期细胞增加,而S期和G2M期细胞减少。表明线粒体途径参与FTY720诱导K562细胞凋亡。
To study the relationship between FTY720-induced apoptosis of K562 cells and mitochondrial pathway. Human leukemia K562 cells were cultured in vitro. Cells were treated with different concentrations of FTY720 for 24 hours. The viability of K562 cells was detected by trypan blue staining. Apoptosis and mitochondrial function were analyzed by flow cytometry. Immunoblotting was used to detect the expression of mitochondrial associated proteins Bcl-2 and Bax. FTY720 cells were collected for 48 h and flow cytometry was used to detect cell cycle. The results showed that with the increase of FTY720 concentration, the survival rate of K562 cells decreased, the apoptosis increased, the mitochondrial membrane potential decreased, and the expression of proapoptotic protein Bax increased significantly. Cell cycle analysis revealed that the number of cells in the Sub G1 phase increased while that in the S phase and G2M phase decreased. The mitochondrial pathway was involved in the apoptosis of K562 cells induced by FTY720.