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目的:通过小鼠骨髓微核试验来研究SNX-2112的遗传毒性。方法:昆明小白鼠尾静脉注射SNX-2112,24 h后处死动物,取双侧股骨制备骨髓细胞悬液,进行骨髓推片和姬姆萨染色,在显微镜下选择细胞分布均匀和着色好的区域阅片,计数含微核嗜多染红细胞(polychromatic erythrocyte,PCE)数。比较各实验组及阳性对照组与阴性对照组的微核率。结果:空白溶媒组及SNX-2112低剂量组与阴性对照相比差别均无显著性(P>0.05),SNX-2112中剂量及SNX-2112高剂量组与阴性对照相比差别有统计意义(P(0.05)。结论:SNX-2112在一定剂量范围内对小鼠染色体无明显的损伤作用。
Objective: To investigate the genotoxicity of SNX-2112 in mouse bone marrow micronucleus test. METHODS: SNX-2112 was injected into caudal vein of Kunming mice. After 24 h, animals were sacrificed. Bone marrow cell suspension was prepared from bilateral femurs. The bone marrow progenitor and Giemsa staining were performed. Under the microscope, the cells were evenly distributed and well colored Reading film, count the number of micronucleated polychromatic erythrocytes (PCE). The micronucleus rate of each experimental group, positive control group and negative control group were compared. Results: There was no significant difference between blank control group and SNX-2112 low-dose group and negative control group (P> 0.05). SNX-2112 medium dose and SNX-2112 high dose group had significant difference compared with negative control group P (0.05) .Conclusion: SNX-2112 has no obvious damage on mouse chromosome in a certain dose range.