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目的:对19株阪崎肠杆菌分离株和1株阪崎肠杆菌标准菌株进行脉冲场电泳(PFGE)分型分析。方法:利用限制性内切酶Xba1对阪崎肠杆菌基因组DNA进行酶切和PFGE分析,并利用BioNumerics软件进行聚类分析。结果:20株阪崎肠杆菌PFGE带型表现出多样性,在可分成16种PFGE型。BQ9、BQ11、BQ12、BQ13和BQ14相似性系数为100%,属于同一PFGE型别。BQ9、BQ11、BQ13和BQ14是同一生产厂家不同品牌的四种产品。其余各分离株和标准菌株的酶切图谱各不相同,相似性系数均在90%以下,被确定为不同PFGE型。结论:PFGE酶切带型与菌株来源有相关性,可应用于阪崎肠杆菌的分子分型和溯源。
OBJECTIVE: To analyze the genotyping of 19 strains of Enterobacter sakazakii and one strain of Enterobacter sakazakii by pulsed-field electrophoresis (PFGE). Methods: Enterobacter sakazakii genomic DNA was digested with restriction endonuclease Xba1 and analyzed by PFGE. BioNumerics software was used for cluster analysis. Results: The 20 strains of Enterobacter sakazakii showed diversity in PFGE bands, which could be divided into 16 PFGE types. BQ9, BQ11, BQ12, BQ13 and BQ14 similarity coefficient of 100%, belong to the same PFGE type. BQ9, BQ11, BQ13 and BQ14 are four different products of the same manufacturer. The rest of the isolates and standard strains of digested maps vary, similarity coefficients were below 90%, was identified as different PFGE type. Conclusion: The PFGE digestion pattern is related to the origin of the strain, and can be applied to the molecular typing and traceability of Enterobacter sakazakii.