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目的探讨邻苯二甲酸二丁酯(DBP)对睾丸支持细胞超微结构和功能的影响。方法将6周龄雄性SD大鼠随机分成0、250、500和1000mg/kg组,每组16只。给予DBP灌胃,分别于染毒2周和4周后各处死8只动物。采用放射免疫法测定血清卵泡刺激素,逆转录聚合酶链反应测定雄激素结合蛋白mRNA和抑制素αmRNA的相对表达量,用透射电镜观察支持细胞的超微结构。结果DBP染毒4周后,卵泡刺激素表现出明显的上升趋势,250和1000mg/kg组与对照组比较差异有统计学意义;精子头计数和每日精子生成量呈现出单一的下降趋势,500和1000mg/kg组与对照组比较差异有统计学意义。雄激素结合蛋白mRNA和抑制素αmRNA表达水平随着DBP染毒剂量的增加呈现出明显的下降趋势,无论是染毒2周还是4周,500和1000mg/kg组与对照组比较差异均有统计学意义。0、250、500和1000mg/kg组的雄激素结合蛋白mRNA相对表达量在染毒2周后分别为0.89±0.15、0.85±0.23、0.54±0.17和0.52±0.16,染毒4周后分别为0.76±0.16、0.73±0.17、0.47±0.14和0.38±0.14;抑制素αmRNA的相对表达量在染毒2周后分别为0.88±0.16、0.61±0.12、0.48±0.15和0.47±0.11,染毒4周后分别为0.75±0.19、0.56±0.16、0.53±0.08和0.45±0.10。电镜观察结果显示,1000mg/kg组支持细胞胞浆中初?
Objective To investigate the effect of dibutyl phthalate (DBP) on the ultrastructure and function of testicular sertoli cells. Methods Six-week-old male SD rats were randomly divided into 0, 250, 500 and 1000 mg / kg groups, 16 in each. Give DBP gavage, respectively, two weeks and four weeks after exposure to poisoning eight animals. Serum follicle stimulating hormone was measured by radioimmunoassay, and the relative expression of androgen-binding protein mRNA and inhibin α mRNA was determined by reverse transcriptase-polymerase chain reaction (RT-PCR). The ultrastructure of the supporting cells was observed by transmission electron microscopy. Results After 4 weeks of DBP exposure, follicle stimulating hormone showed a marked upward trend. There was a significant difference between 250 mg / kg and 1000 mg / kg group and the control group. Sperm count and daily sperm production showed a single decreasing trend. The difference between the 500 and 1000 mg / kg groups and the control group was statistically significant. Androgen binding protein mRNA and inhibin αmRNA expression levels with DBP exposure dose showed a significant downward trend, either for 2 weeks or 4 weeks exposure, 500 and 1000mg / kg group compared with the control group were statistically significant Significance of learning. The relative expression levels of androgen-binding protein mRNA in the 0, 250, 500 and 1000 mg / kg groups were 0.89 ± 0.15, 0.85 ± 0.23, 0.54 ± 0.17 and 0.52 ± 0.16 after 2 weeks of exposure, respectively 0.76 ± 0.16, 0.73 ± 0.17, 0.47 ± 0.14 and 0.38 ± 0.14 respectively. The relative expression of inhibin α mRNA was 0.88 ± 0.16, 0.61 ± 0.12, 0.48 ± 0.15 and 0.47 ± 0.11 respectively after 2 weeks of exposure, 0.75 ± 0.19, 0.56 ± 0.16, 0.53 ± 0.08 and 0.45 ± 0.10 respectively after the week. Electron microscopy results showed that 1000mg / kg group of supporting cells in the cytoplasm first?