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为了明确中国春谷子waxy基因序列的变异,本研究以引进的53份谷子品种(系)为材料,采用PCR产物测序,对waxy基因exon2-exon4序列进行比对分析。研究结果显示:非糯、低直链淀粉含量和糯质类型频率分别为22.6%、54.7%和9.4%;在非糯类型中,11、12、22、23号材料检测到碱基缺失和置换,1、13、18和27号仅存在碱基缺失;在低直链淀粉类型中,9、17和24号同时存在碱基插入、缺失和置换,14和15号品种为碱基缺失和置换,大红谷(00009836)为碱基插入和缺失,20号和43号仅分别出现置换和缺失;在糯质类型中,红粘谷(00000537)同时检测到碱基插入、缺失和置换,杏黄粘存在缺失和置换。根据exon2-exon4区间序列变异,以阈值为78将53份材料聚为十大类,其中第一类品种数量较多,第五类群SNP多态性较丰富。
In order to clarify the variation of the waxy gene sequence of Chinese Spring Millet, we analyzed the exon2-exon4 sequence of waxy gene by using 53 introduced millet varieties (lines) as materials. The results showed that the content of non-waxy, low amylose and waxy type were 22.6%, 54.7% and 9.4% respectively. In the non-waxy type, 11,12,22,23 base deletions and substitutions 1, 13, 18, and 27, respectively. In the low amylose types, base insertions, deletions and substitutions exist in Nos. 9, 17 and 24, and base deletions and substitutions in varieties 14 and 15 , Dahong Valley (00009836) was a base insertion and deletion, only 20 and 43 were replaced and deleted, respectively. In the waxy type, Hongyin Valley (00000537) also detected base insertion, deletion and substitution, There is a lack of and replacement. According to the sequence variation of exon2-exon4, 53 materials were grouped into ten categories with a threshold value of 78, of which the first type was more in number and the fifth group was more abundant in SNP.