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目的:对大肠腺瘤组、LST良性组、大肠腺癌组分别进行Survivin基因表达的研究,为进一步完善大肠肿瘤发生的分子生物机制奠定基础。方法:分别用RT-PCR法和免疫组化SP法对大肠腺瘤、大肠腺癌、LST良性组及正常大肠黏膜组织,检测Sur-vivin的表达。结果:大肠腺癌组、大肠腺瘤组和LST良性组中Survivin表达显著高于正常大肠黏膜组(P<0.05);大肠腺瘤组和LST良性组Survivin表达低于大肠癌组(P<0.05),而大肠腺瘤组和LST良性组间无差异表达。结论:Survivin的过度表达是大肠上皮癌变过程的早期事件之一。LST与大肠腺瘤有着相同的基因改变,同样是由于多种基因协同作用的结果,同样经过腺瘤-癌顺序学说演变成大肠腺癌。
OBJECTIVE: To investigate the expression of Survivin gene in colorectal adenoma, LST benign and colorectal adenocarcinoma respectively, which lays the foundation for further improving the molecular biological mechanism of colorectal tumor. Methods: The expressions of Sur-vivin in colorectal adenoma, colorectal adenocarcinoma, LST positive group and normal colorectal mucosa were detected by RT-PCR and immunohistochemistry respectively. Results: The expression of Survivin in colorectal adenocarcinoma, colorectal adenoma and LST benign group was significantly higher than that in normal colorectal mucosa (P <0.05). The expression of Survivin in colorectal adenoma group and LST benign group was lower than that in colorectal cancer group (P <0.05) ), While no significant difference was found between colorectal adenoma group and benign LST group. Conclusion: Survivin overexpression is one of the early events of colorectal carcinogenesis. LST and colorectal adenomas have the same genetic changes, the same is due to the synergy of a variety of genes, also through adenoma - cancer sequence theory evolved into colon adenocarcinoma.