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为了探讨日粮中加入菜籽油和猪油调和油对小鼠肝脏PPARα基因表达的影响,试验将雄性KM小鼠随机分成正常剂量组、高剂量组,分别在日粮中添加3.8%和6.5%调和油,8周后称量体重,取完整肝脏组织,计算肝指数,H.E.染色进行病理观察,实时荧光定量RT-PCR检测肝脏PPARα水平。结果表明:与正常剂量组比较,高剂量组小鼠肝脏病理剖检无眼观病理变化,体重、肝功能无统计学意义(P>0.05),肝脏PPARα水平极显著上升(P<0.01)。说明饮食中过量摄入菜籽油和猪油调和油极显著上调小鼠肝脏PPARα的表达。
In order to investigate the effects of adding rapeseed oil and lard oil on the PPARα gene expression in liver of mice, male KM mice were randomly divided into normal dose group and high dose group, and 3.8% and 6.5% % Reconcile oil. After 8 weeks, the body weight was weighed and the whole liver tissue was taken out to calculate the liver index. HE staining was used to observe the pathological changes. The level of PPARα in the liver was detected by real-time fluorescence quantitative RT-PCR. The results showed that compared with the normal dose group, there was no significant difference in the pathological changes of the liver, body weight and liver function in the high dose group (P> 0.05). The level of PPARα in the liver increased significantly (P <0.01). The results showed that excessive dietary intake of rapeseed oil and lard oil greatly increased the expression of PPARα in the liver of mice.