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目的:考察阿托品光学异构体的毛细管电泳拆分方法。方法:采用毛细管电泳法,弹性石英毛细管柱(60cm×75"m,有效长度40cm),磷酸盐缓冲液浓度:30mmol·L~(-1),重力进样高度差10cm,进样时间:5s,检测波长:225nm。分别对手性拆分剂种类及浓度、缓冲溶液的pH、运行电压、有机溶剂等进行考察,优选出最佳拆分条件。结果:阿托品光学异构体最佳拆分条件为:缓冲液pH为7.0,磺酸化-β-环糊精聚合物(S-β-CDP)浓度为10mg·ml~(-1),电压为12kV。结论:本方法简便、快速,能实现对阿托品光学异构体的拆分。
OBJECTIVE: To investigate capillary electrophoresis resolution of atropine optical isomers. Methods: The capillary electrophoresis was used to measure the difference of the height of gravity injection between 10 cm and 30 cm, the concentration of phosphate buffer was 60 cm × 75 cm, the effective length was 40 cm, the injection time was 5s, detection wavelength: 225nm.According to the types and concentrations of chiral resolving agents, the pH value of buffer solution, operating voltage and organic solvent, respectively, the optimal resolution conditions were optimized.Results: The optimum resolution of atropine optical isomers The conditions were as follows: buffer pH was 7.0, S-β-CDP concentration was 10 mg · ml -1, voltage was 12 kV.Conclusion: The method is simple, rapid, Achieve the resolution of the optical isomers of atropine.