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目的探讨胃癌组织中EB病毒(EBV)感染与抑癌基因PTEN的关系。方法选择胃癌芯片有效组织476例,用原位杂交法检测胃癌组织中EBV编码的小RNA(EBERs),分析EBV相关胃癌病理形态及临床特征;用免疫组化EnVision法检测核PTEN蛋白表达,分析EBV和核PTEN在胃癌的表达与患者淋巴结转移、Lauren′s分型的关系。结果 476例胃癌中检测出EBV感染18例(3.8%),其中的14例伴淋巴结转移癌,所有癌细胞均表达EBERs,EBV相关胃癌分化程度低。核PTEN在胃癌组织中的表达率为17.0%(81/476);核PTEN与EBV感染胃癌呈负相关。结论 EBV相关胃癌具有独特的临床病理特征,胃癌组织中的抑癌基因核PTEN对判断胃癌的恶性程度和估计预后有一定意义。
Objective To investigate the relationship between Epstein-Barr virus (EBV) infection and tumor suppressor gene PTEN in gastric cancer. Methods Forty-six patients with gastric cancer were selected for effective organization of gastric cancer. EBV-encoded small RNAs (EBERs) were detected by in situ hybridization in situ hybridization. The pathological features and clinical features of EBV-related gastric cancer were analyzed. The expression of nuclear PTEN protein was detected by EnVision immunohistochemistry. EBV and nuclear PTEN expression in gastric cancer and lymph node metastasis, Lauren’s classification. Results Eighteen cases (3.8%) of EBV infection were detected in 476 cases of gastric cancer, of which 14 cases were accompanied with lymph node metastasis, all of them expressed EBERs, EBV-related gastric cancer had a low degree of differentiation. The expression rate of nuclear PTEN in gastric cancer tissues was 17.0% (81/476). Nuclear PTEN was negatively correlated with EBV-infected gastric cancer. Conclusions The EBV-associated gastric cancer has unique clinical and pathological features. The tumor suppressor gene nuclear transfection of PTEN in gastric cancer has certain significance in judging the malignant degree of gastric cancer and estimating the prognosis.