小麦（ＴｒｉｔｉｃｕｍａｅｓｔｉｖｕｍＬ．）耐酸性土壤的遗传改良途径之一是由其它基因库转入耐Ａ１基因。以Ａｔｌａｓ６６作为耐Ａ１资源，通过苏木精评价法选出了硬红冬（ＨＲＷ）小麦的耐Ａ１近等基因系，目的是鉴定这些品系中的耐Ａ１基因及其在人工液体培养和含Ａ１的自然土壤条件下的遗传表达情况。用Ｃｈｉｓｈｏｌｍ和Ｃｅｎｔｕｒｙ的耐Ａ１及Ａ１敏感近等基因系进行了实验室和生长室试验。将耐Ａ１等基因系与其轮回亲本杂交，对杂交后代进行苏木精污染；群体污染等级的分离结果表明，从Ａｔｌａｓ６６中转入了单个显性耐Ａ１基因。这一基因似乎与２１８０携带的耐Ａ１基因不同，在培养液中测得的相对根长（ＲＲＬ）的剂量反应曲线进一步证实了由苏木精评价法预测出的耐Ａ１性差异，随着Ａ１胁迫浓度的提高（０．０９～０．７２ｍＭＡ１），每个耐Ａ１等基因系的ＲＲＬ均呈直线型降低，而Ａ１敏感等基因系和轮回亲本的ＲＲＬ则呈曲线型反应。在５种酸性土壤样品中的试验结果表明，耐Ａ１等基因系的ＲＲＬ比它们的轮回亲本高１９％（Ｃｈｉｓｈｏｌｍ）和３８％（Ｃｅｒｔｕｒｙ）。总之，苏木精污染法可以用于评价基因型的耐Ａ１性；而来自Ａｔｌａｓ６６的耐Ａ１基因不能完全表达，或者? One of the genetic improvements of acid-tolerant soils for wheat (Triticum aestivum L.) is the transfer of genes from other gene pools to the A1-resistant gene. Atlas66 was used as a resistance to A1 resource and the A1-resistant proximal isogenic lines of hard red winter wheat (HRW) were selected by hematoxylin-eosin assay in order to identify the resistance to A1 gene in these lines and their application in artificial liquid culture The natural soil conditions of the genetic expression. Laboratory and growth chamber experiments were conducted using Chisholm and Century resistant A1 and A1 sensitive isogenic lines. The resistant A1 gene was crossed with its reincarnated parents to hematoxylin-contaminated hybrid progeny. The segregation of population pollination grade showed that a single dominant-resistance A1 gene was transferred from Atlas66. This gene appears to be distinct from the A1-resistant gene carried by 2180 and the dose-response curve of the relative root length (RRL) measured in the broth further confirms the difference in A1 tolerance predicted by the hematoxylin-eosin assay, The increase of stress concentration (0.09 ~ 0.72mMA1) showed a linear decrease of RRL in each of the resistant A1 isogenic lines, while the RRL of A1 sensitive isogenic lines and recurrent parents showed a curvilinear response. The results of the tests in five acid soil samples showed that the RRL of the resistant A1 isogenic lines were 19% higher (Chisholm) and 38% (Certury) higher than their recurrent parents. In summary, hematoxylin contamination can be used to assess A1-resistance of genotypes; whereas A1-resistant genes from Atlas66 can not be fully expressed, or