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天花粉蛋白(Trichosanthin,TCS)制备成免疫毒素后,可抑制肿瘤细胞的生长,已尝试用于治疗恶性肿瘤。在无细胞体系中TCS可通过抑制蛋白质的合成发挥毒性作用。对完整的活细胞,近来发现它可阻断激活信号传递中ZAP-70与CD3ζ链的物理结合,并能诱导人体外周血淋巴细胞发生凋亡。凋亡为一受基因控制的细胞程序性死亡,有关死亡信号传递通路中相关基因的分离是研究和阐明这一细胞自杀事件的有效途径,并有助于了解TCS的作用机理和探索其用于恶性肿瘤治疗的新方式。本文采用mRNA差别显示技术,从TCS诱导
Trichosanthin (TCS), after being prepared into immunotoxins, can inhibit the growth of tumor cells and has been tried to treat malignant tumors. In cell-free systems, TCS can exert its toxic effects by inhibiting protein synthesis. For intact living cells, it has recently been found that it blocks the physical binding of ZAP-70 to the CD3ζ chain during activation signaling and induces apoptosis in human peripheral blood lymphocytes. Apoptosis is a gene-controlled programmed cell death. The isolation of related genes in the death signal transmission pathway is an effective way to study and elucidate this cell suicide event, and helps to understand the mechanism of TCS and explore its use. New ways to treat cancer. This article uses mRNA differential display technology, induced from TCS