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目的观察芽囊原虫在不同培养基中的生长状况,筛选合适的芽囊原虫培养方法。方法将10份芽囊原虫阳性粪便分别接种至Jone’s液、vitro液、1640 3种培养基中培养,选取生长情况良好、虫密度高的一份粪便传代培养后等量接种至3种不同的培养基中,每24 h计数虫体数量一次,连续观察10 d,并观察芽囊原虫的形态学变化和生长情况。结果10份粪便分别接种至3种培养基中培养,48 h后发现1640和Jone’s液中的虫密度高于vitro液。同一份粪便接种至3种不同培养基中连续观察10 d,结果表明3种培养基中虫体生长呈规律变化,均在接种后3、6、9 d出现生长高峰;Jone’s液中芽囊原虫密度最高。vitro液中观察到的虫体形态最清晰,且富有活力;Jone’s液中能观察到虫体多种繁殖状态。结论Jone’s液适于芽囊原虫的生长繁殖,可作为首选培养基;vitro液可作为观察芽囊原虫生长情况的首选培养基。
Objective To observe the growth of Blastocystis protozoa in different culture media and screen the appropriate Blastocystis protozoal culture method. Methods Ten positive strains of Blastulas were inoculated into Jone’s liquid and 16403 in vitro respectively. One fecal culture with good growth was selected and subcultured into three different cultures The number of worms was counted once every 24 h for 10 days. The morphological changes and growth of Blastocystis protozoa were observed. Results Ten stools were inoculated into three kinds of culture medium respectively. After 48 h, the pest density of 1640 and Jone’s liquid was higher than that of the other liquid. The same stool was inoculated into three different media for 10 consecutive days. The results showed that the growth of the three kinds of culture media changed regularly, with the peak of growth on the 3rd, 6th and 9th day after inoculation. The highest density. The morphology of the parasites observed in vitro was the clearest and full of vitality. The multiplication of parasites in Jone’s solution was observed. Conclusion Jone’s liquid is suitable for the growth and reproduction of Blastocystis protozoa and can be used as the preferred culture medium. The vitro liquid can be used as the preferred medium for observing the growth of Blastocystis protozoa.