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对球孢白僵菌(Beauveria bassiana(Bais.)Vuill)D1-5菌株的3种分生孢子气生孢子、芽生孢子和深层孢子进行电镜观察,并利用双向凝胶电泳技术对3种孢子的特异性表达蛋白进行分析与筛选。结果显示,电镜下气生分生孢子呈球形,外周有棒状结构包裹,具有明显的疏水特性;芽生孢子为圆柱形,大小不均一,表面光滑;深层孢子球形透明,大小均一,表面粗糙,3种分生孢子孢子壁厚度差异不明显。经双向凝胶电泳对3种分生孢子进行特异性表达蛋白分离后,使用PDquest软件对结果进行分析,深层分生孢子与芽生分生孢子蛋白表达图谱相比较,有20个蛋白质表达量发生2倍以上改变,其中8个蛋白质表达量上调,12个蛋白质表达量下调;气生分生孢子与芽生分生孢子蛋白表达图谱相比较,有57个蛋白质表达量发生2倍以上改变,其中43个蛋白质表达量上调,14个蛋白质表达量下调;深层分生孢子与气生分生孢子蛋白表达图谱相比较,有66个蛋白质表达量发生2倍以上改变,其中8个蛋白质表达量上调,58个蛋白质表达量下调。
Three species of conidia, conidia and conidia of conidia of Beauveria bassiana (Bais.) Vuill were observed under electron microscope. The distribution of three conidia, Specific expression of proteins for analysis and screening. The results showed that the aerial conidia were spherical in shape and surrounded by a rod-like structure. The conidiospores were cylindrical with uniform size and smooth surface. The deep sporophytes were spherical, uniform in size and rough in surface. 3 Species conidia spore wall thickness difference is not obvious. Two kinds of conidial proteins were separated by two-dimensional gel electrophoresis, and the results were analyzed by using PDquest software. Compared with the protein expression profiles of conidia and buds of conidia, 20 proteins were expressed 2 Fold change, of which 8 proteins were up-regulated and 12 proteins were down-regulated. Compared with the protein expression profiles of aerial conidia and buds, 57 proteins changed more than twice, of which 43 Upregulation of protein expression, down-regulation of 14 proteins; compared with the protein expression profiles of the aerial conidia, 66 proteins were more than twice as much, of which 8 were up-regulated, 58 Protein expression decreased.