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为了探究乙醇中添加Fe2+或Mn2+对土壤生物消毒效果的影响,完善低浓度乙醇溶液土壤消毒的工艺参数,为乙醇溶液消毒土壤应用于实际生产提供理论依据和技术支持。采用密封塑料瓶模拟耕层土壤的方式,以接种尖孢镰刀菌的土壤为研究对象,3%乙醇溶液消毒土壤为对照,研究添加质量比为0.001%、0.010%、0.100%的Fe2+或Mn2+的3%乙醇溶液对土壤中尖孢镰刀菌的数量、杀灭率的影响。结果表明:添加Fe2+的3%乙醇溶液消毒效果改善,其中以添加Fe2+浓度为0.001%的3%乙醇溶液效果最好,在处理11d后杀灭率达到97.95%,且与对照组存在显著差异;添加Mn2+的3%乙醇溶液消毒效果也有所改善,其中以添加Mn2+浓度为0.100%的3%乙醇溶液效果最好,在处理11d后杀灭率达到98.74%,且与对照组存在显著差异。说明在实际生产中采用添加0.001%Fe2+的3%乙醇溶液进行土壤生物消毒即可达到显著的杀菌效果,并改善土壤环境。
In order to explore the effect of adding Fe2 + or Mn2 + on the sterilizing effect of soil microorganisms in ethanol, the process parameters of soil disinfection in low concentration ethanol solution were perfected to provide the theoretical basis and technical support for the practical production of ethanol solution disinfecting soil. Using sealed plastic bottle to simulate the topsoil, the soil inoculated with Fusarium oxysporum was taken as the research object and the 3% ethanol solution was used as the control to study the effect of adding Fe2 + or Mn2 + with mass ratio of 0.001%, 0.010% and 0.100% Effect of 3% ethanol solution on the number and killing rate of Fusarium oxysporium in soil. The results showed that the disinfection effect of 3% ethanol solution with Fe2 + was improved. Among them, 3% ethanol solution with Fe2 + concentration of 0.001% was the best, and the killing rate was 97.95% after 11 days of treatment, which was significantly different from the control group. The disinfection effect of 3% ethanol solution with Mn2 + also improved. Among them, 3% ethanol solution with Mn2 + concentration of 0.100% was the best, killing rate was 98.74% after 11 days of treatment, and there was significant difference with the control group. It shows that in the actual production, adding 3% ethanol solution of 0.001% Fe2 + for soil biological disinfection can achieve significant bactericidal effect and improve the soil environment.