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目的探讨CK10、CK14、CK17、P16在宫颈不成熟鳞状化生(IM)及宫颈上皮内瘤变(CIN)中的表达和意义。方法应用SP免疫组化法检测CK10、CK14、CK17、P16在宫颈45例IM及120例CIN标本中的表达情况,并对结果进行分析。结果 CK17、CK10在宫颈IM中的阳性表达率分别为100%、95.6%,明显高于LSIL和HSIL组(分别为CK17:11.3%、15%;CK10:20.0%、22.5%。P均<0.01);P16在LSIL和HSIL中的表达率均为100%,明显高于IM组(13.3%,P<0.01);CK14在IM组、LSIL和HSIL组的表达率分别为64.4%、61.2%、67.5%,三组间差别不显著(P>0.05);在LSIL和HSIL组,CK14、P16的表达率无明显差别,但在LSIL中,阳性细胞位于上皮下1/3层,在HSIL中,位于上皮上1/3或全层。结论①CK17、CK10、P16在宫颈IM和CIN病变中表达具有明显差异,有助于两种病变的鉴别诊断;②CK14、P16可用于LSIL和HSIL的辅助鉴别诊断。
Objective To investigate the expression and significance of CK10, CK14, CK17 and P16 in immature cervical squamous metaplasia (IM) and cervical intraepithelial neoplasia (CIN). Methods The expression of CK10, CK14, CK17 and P16 in 45 cases of cervical and 120 cases of CIN specimens were detected by SP immunohistochemical method, and the results were analyzed. Results The positive rates of CK17 and CK10 in cervical IM were 100% and 95.6%, respectively, which were significantly higher than those in LSIL and HSIL (CK17: 11.3% and 15%, CK10: 20.0% and 22.5%, P <0.01 respectively) ). The positive rate of P16 in LSIL and HSIL was 100%, which was significantly higher than that in IM (13.3%, P <0.01). The expression rates of CK14 in IM, LSIL and HSIL were 64.4% and 61.2% 67.5%, there was no significant difference between the three groups (P> 0.05). In LSIL and HSIL groups, there was no significant difference in the expression rates of CK14 and P16, but in LSIL, Located on the epithelium 1/3 or full-thickness. Conclusions ①CK17, CK10, P16 expression in cervical IM and CIN lesions have significant differences, contribute to the differential diagnosis of two lesions; ② CK14, P16 can be used for the differential diagnosis of LSIL and HSIL.