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目的:探讨靶向溶酶体药物氯喹对非小细胞肺癌A549细胞对紫杉醇的化疗敏感性的影响。方法:以紫杉醇及氯喹作用于A549细胞,Lyso-Tracker Red DND-99进行细胞内溶酶体染色,采用荧光显微镜观察细胞内溶酶体变化,应用MTT比色法比较药物对A549细胞的生长抑制作用。结果:在紫杉醇作用的A549细胞浆内可观察到大量的点灶状红色荧光囊泡,而在氯喹及联合紫杉醇作用后红色荧光囊泡数量显著减少并伴囊泡体积增大及囊泡内弥散荧光染色,氯喹使得紫杉醇对A549细胞的IC_(50)从6.815μmol/L下降至2.611μmol/L,氯喹显著提高紫杉醇对A549细胞的生长抑制作用。结论:氯喹靶向溶酶体提高A549细胞对紫杉醇的化疗敏感性。
Objective: To investigate the effect of targeted lysosomal drug chloroquine on chemosensitivity of paclitaxel in non-small cell lung cancer A549 cells. Methods: A549 cells were treated with paclitaxel and chloroquine. Intracellular lysosomes were stained with Lyso-Tracker Red DND-99. The changes of intracellular lysosomes were observed by fluorescence microscopy. The growth inhibition of A549 cells was compared by MTT assay effect. Results: A large number of focal red fluorescent vesicles were observed in A549 cells treated with paclitaxel. However, the number of red fluorescent vesicles decreased significantly with the increase of vesicle volume and vesicle dispersion Fluorescence staining and chloroquine decreased the IC 50 of paclitaxel to A549 cells from 6.815 μmol / L to 2.611 μmol / L, and chloroquine significantly enhanced the growth inhibition of paclitaxel to A549 cells. CONCLUSION: Chloroquine targeting lysosomal enhances chemosensitivity of paclitaxel in A549 cells.