采用葡聚糖包被活性炭技术处理小牛血清，培养喉癌细胞株Ｈｅｐ┐２，观察该细胞ｒａｓ基因表达对睾酮（Ｔ）、１７β┐雌二醇的剂量依赖性。结果表明：该细胞为性激素敏感细胞，当１７β┐雌二醇浓度为１０－８ｍｏｌ／Ｌ时对ｒａｓ基因的表达有明显的抑制作用，表达率为１１．１１％；而睾酮浓度为１０－９时对ｒａｓ基因的表达率增至４４．４４％。此结论为喉癌的内分泌治疗提供了理论基础。 The dextran-coated activated carbon was used to treat calf serum, and the laryngeal carcinoma cell line Hep┐2 was cultured to observe the dose-dependent effect of ras gene expression on testosterone (T) and 17β┐ estradiol. The results showed that the cells were sensitive to sex hormones. When the concentration of 17β┐ estradiol was 10-8 mol / L, the expression of ras gene was inhibited obviously, the expression rate was 11.11%, while the testosterone concentration was 10-9 Ras gene expression rate increased to 44.44%. This conclusion provides a theoretical basis for endocrine therapy of laryngeal cancer.