目的:观察胰岛素受体在猕猴脊髓腰段第4~6(L4~L6)节段及相应节段背根神经节(dorsal root ganglion,DRG)中的形态学分布特点。方法:取1只成年猕猴的L4~L6节段脊髓并留取相应节段的DRG置于固定液中。脊髓冷冻水平切片(30μm)平均分为4组,分别行Nissl染色、荧光免疫组织化学染色、免疫组织化学染色和对照组处理,再于光学显微镜下观察;DRG平均分为2组,分别行免疫组织化学染色和对照组处理,再于光学显微镜下观察。结果:胰岛素受体分布于猕猴腰段脊髓L4~L6节段及相应节段的DRG中:(1)在腰段脊髓L4~L6节段,背角可见胰岛素受体阳性神经元胞体及终末,并大量分布于背角浅层(集中在第I、II层),腹角也可观察到散在胰岛素受体阳性神经元;(2)在L4~L6节段对应的DRG中,胰岛素受体阳性产物主要集中分布于中型(20μm<直径≤40μm)、小型(直径≤20μm)神经元。结论:本研究为进一步研究灵长类动物脊髓和DRG中胰岛素受体的功能提供形态学依据。 OBJECTIVE: To observe the morphological distribution of insulin receptor in the fourth to sixth (L4 ~ L6) and corresponding segmental dorsal root ganglion (DRG) of the spinal cord of the rhesus monkey. Methods: One adult adult Rhesus monkey L4 ~ L6 spinal cord was taken and DRG of the corresponding segment was placed in the fixative solution. The spinal cord frozen sections (30μm) were equally divided into 4 groups and were subjected to Nissl staining, fluorescent immunohistochemical staining, immunohistochemical staining and control group, respectively, and observed under light microscope. The DRGs were divided into 2 groups, Histochemical staining and control group, and then observed under a light microscope. Results: Insulin receptors were distributed in the DRGs of L4 ~ L6 segments and corresponding segments in the lumbar spinal cord of macaque: (1) In the L4 ~ L6 segments of the lumbar spinal cord, insulin receptor positive neurons and terminal , Scattered in the superficial layers of the dorsal horn (concentrated in the first and the second layer), scattered insulin receptor positive neurons were observed in the ventral horn. (2) In the DRGs corresponding to the L4 ~ L6 segments, the insulin receptor Positive products mainly concentrated in medium (20μm