Objective:To evaluate the antibacterial activity of the methanolic extract of mango(Mangifera indica L) seed kernel.Methods:Chokanan mango seed kernel and seed kernels from assorted mango varieties were collected,cleaned,dried and powered.Crude methanolic extracts of mango seed kernel were analyzed for the phytochemical constituents.The free radical scavenging activity was determined by 2,2-Diphenyl-1-picrylhydrazyl(DPPH) assay.Antibacterial activity was evaluated by disc diffusion assay with three medically important bacterial pathogens such as methicillin resistant Staphylococcus aureus(S.aureus)(MRSA),Escherichia coli(E.coli) and Vibrio vulnificus(V.vulnificus).Results:Qualitative phytochemical analysis indicated the presence of important phytochemical compounds such as glycosides,saponins,flavanoids, tannins and alkaloids.There was no significant difference in the phytochemical content between the single and assorted mango seed kernels.However,the free radical scavenging study indicated that the assorted mango kernels showed slighdy higher activity than the single species(P<0.05). The crude methanolic extract of mango seed kernel at a concentration of 100 mg/mL is found to have potential antimicrobial activity against MRSA and E.coli compared to V.vulnificus. Study on the antibacterial activity also indicated that there was no significant difference in the antibacterial activity of the single and assorted mango seed kernel extracts.Conclusions:The present study conclusively demonstrates the free radical scavenging activity and antibacterial activities of mango seed kernel.In addition,the results also indicated that there is no significant difference in the phytochemical content and biological activity of mango kernels from single and assorted mango varieties. Objective: To evaluate the antibacterial activity of the methanolic extract of mango (Mangifera indica L) seed kernel. Methods: Chokanan mango seed kernel and seed kernels from assorted mango varieties were collected, cleaned, dried and powered. Crude methanolic extracts of mango seed kernel was analyzed for the phytochemical constituents. The free radical scavenging activity was determined by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay. Antibacterial activity was evaluated by disc diffusion assay with three medically important bacterial pathogens such as methicillin resistant Staphylococcus aureus S. aureus) (MRSA), Escherichia coli (E. coli) and Vibrio vulnificus (V. vulgaris). Results: Qualitative phytochemical analysis indicated the presence of important phytochemical compounds such as glycosides, saponins, flavanoids, tannins and alkaloids. Was was no significant difference in the phytochemical content between the single and assorted mango seed kernels.However, the free radical scavenging study indi cated that the assorted mango kernels showed slighdy higher activity than the single species (P <0.05). The crude methanolic extract of mango seed kernel at a concentration of 100 mg / mL is found to have potential antimicrobial activity against MRSA and E. coli to V. vulnificus. Study on the antibacterial activity also indicated that there was no significant difference in the antibacterial activity of the single and assorted mango seed extracts. Conclusions: The present study conclusively demonstrates the free radical scavenging activity and antibacterial activities of mango seed kernel.In addition, the results also indicates that there is no significant difference in the phytochemical content and biological activity of mango kernels from single and assorted mango varieties.