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目的探究脐血干细胞诱导分化为多巴胺能神经元对大鼠的行为学测试在细胞移植治疗帕金森病(PD)的研究分析。方法选取医院动物中心提供的大鼠,构建PD大鼠模型后,选取17只移植了脐血干细胞诱导的多巴胺能神经元大鼠作为研究组,同时选取17只注射0.9%氯化钠注射液的大鼠作为对照组。1对比两组大鼠在移植前1 d与细胞移植后的1 d、3 d、1周、2周、1个月、3个月和6个月时进行腹腔内阿朴吗啡注射后旋转数;2在进行细胞移植后的1 d、3 d、1周、2周、1个月、3个月和6个月时,检测多巴胺的代谢产物如DOPAC和HVA,以了解纹状体和黑质多巴胺的代谢情况;3将人脐血源性的干细胞注入大鼠腋区皮下和脑内观察其体内成瘤能力。结果 1研究组大鼠在移植前与移植后旋转圈数明显减少,而且随着时间的推移有下降趋势;对照组大鼠移植前后并无明显减少现象,两组比较差异有统计学意义(P<0.05);2主要检测DOPAC和HVA,测试代谢结果较稳定;3研究组PD大鼠移植由脐血干细胞诱导分化后的多巴胺能神经元后,无形成畸形胎瘤的风险,安全性较高。结论本研究成功地找到了廉价高效的脐血干细胞诱导分化为多巴胺能神经元的方法,并使PD大鼠症状减轻,PD大鼠行为明显得到改善,说明诱导分化的多巴胺能神经元具有初步的临床效果,具有进一步推广的意义。
OBJECTIVE: To investigate the behavioral testing of rat umbilical cord blood stem cells induced to differentiate into dopaminergic neurons in the study of cell transplantation for the treatment of Parkinson’s disease (PD). Methods The rats from the hospital animal center were selected to establish a rat model of PD. Seventeen rats were injected with umbilical cord blood stem cells induced by umbilical cord blood stem cells as study groups. Seventeen mice injected with 0.9% sodium chloride injection Rats served as control group. 1 Comparison of the number of rotations after 1 day, 3 days, 2 weeks, 1 month, 3 months and 6 months after the transplantation of apomorphine 1 day before transplantation and 2 days after transplantation ; 2 Dopamine metabolites such as DOPAC and HVA were tested at 1 d, 3 d, 1 week, 2 weeks, 1 month, 3 months and 6 months after cell transplantation to understand the presence of striatum and black Dopamine metabolism; 3 human umbilical cord blood stem cells injected into the axillary area of the rat subcutaneously and brain to observe its in vivo tumorigenic ability. Results 1 The number of rotations of the study group before and after transplantation decreased significantly, and with the passage of time there was a downward trend; there was no significant reduction in the control group rats before and after transplantation, the difference between the two groups was statistically significant (P <0.05) .2 The main test of DOPAC and HVA, the test results of metabolites more stable; 3 study group PD rats induced by umbilical cord blood stem cells after differentiation of dopaminergic neurons, no formation of deformity of the risk of benign tumor, high safety . Conclusions This study successfully found a cheap and efficient method for inducing differentiation of umbilical cord blood stem cells into dopaminergic neurons, and alleviated the symptoms of PD rats and the behavior of PD rats was significantly improved, indicating that the induced differentiation of dopaminergic neurons has a preliminary Clinical effect, with the significance of further promotion.