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目的 :探讨骨髓单个核细胞植入心肌梗死及其周边区对心肌梗死的治疗作用 ,促进血管新生及相关基因表达情况。方法 :以冷冻损伤的方法建立新西兰种家兔心梗模型 ,4周后 ,将提取分离的骨髓单个核细胞 ,植入心梗及心梗周边区。分析心梗及心梗周边区病理组织学特点 ,比较区域内微血管密度 ,并以Westernblot研究骨髓单个核细胞移植对心梗及心梗周边区血管生成相关基因表达的影响。结果 :(1)骨髓单个核细胞植入心梗及心梗周边区减少移植区域瘢痕化 ,增加微血管密度 ;(2 )骨髓单个核细胞植入心梗及心梗周边区使某些重要血管生成相关基因 ,VEGF ,FGF及Angiopoietin -Ⅰ表达上调 ,于心梗周边区作用更加显著。结论 :骨髓单个核细胞植入心梗及心梗周边区可以减少移植区域瘢痕化 ,增加微血管密度 ,上调心梗及心梗周边区重要血管生成相关基因。
OBJECTIVE: To investigate the therapeutic effect of bone marrow mononuclear cells implanted into myocardial infarction and its peripheral area on myocardial infarction and to promote angiogenesis and related gene expression. Methods: New Zealand rabbit model of myocardial infarction was established by freezing injury. After 4 weeks, isolated mononuclear cells were isolated and implanted into myocardial infarction and peripheral region of myocardial infarction. The histopathological characteristics of MI and MI were analyzed to compare the microvessel density in the region. The effects of bone marrow mononuclear cell transplantation on the gene expression of angiogenesis in MI and MI were studied by Western blotting. Results: (1) Bone marrow mononuclear cells were implanted into the peripheral area of myocardial infarction and myocardial infarction to reduce scarring of transplanted area and increase microvessel density. (2) Bone marrow mononuclear cells implanted into myocardial infarction and peripheral area of myocardial infarction made certain important angiogenesis Related genes, VEGF, FGF and Angiopoietin-Ⅰ expression increased in the peripheral area of myocardial infarction even more significant. CONCLUSION: Bone marrow mononuclear cells implanted into myocardial infarction and peri-infarct border area can reduce scarring of transplanted area, increase microvessel density and up-regulate important angiogenesis related genes in myocardial infarction and myocardial infarction area.