Inhibitors of 5-lipoxygenase inhibit expression of intercellular adhesion molecule-1 in human melano

来源 :Acta Pharmacologica Sinica | 被引量 : 0次 | 上传用户:clxzzx
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AIM: To study the effect of 5-lipoxygenase inhibitors on the expression of intercellular adhesion molecule-1 (ICAM-1) inmelanoma cells. METHODS: ICAM-1 protein of human melanoma cell a375 was detected by enzyme-linkedimmunosorbent, flow cytometry and Western blot analysis. Level of ICAM-1 mRNA in a375 was evaluated byNorthern blot analysis. Adhesion of a375 to endothelial cell EC304 was analyzed by isotopic tracing. RESULTS:5-Lipoxygenase inhibitors nordihydroguaiaretic acid, AA861 and MK886, could suppress the expression of ICAM-1protein as well as of its mRNA in a375 cells and reduce the adhesion of a375 to EC304. CONCLUSION:5-Lipoxygenase inhibitors can inhibit the expression of ICAM-1 in human melanoma cells and may be valuable fortreatment of melanoma metastasis. AIM: To study the effect of 5-lipoxygenase inhibitors on the expression of intercellular adhesion molecule-1 (ICAM-1) inmelanoma cells. METHODS: ICAM-1 protein of human melanoma cell a375 was detected by enzyme-linked immunosorbent, flow cytometry and Western Adhesion of a375 to endothelial cell EC304 was analyzed by isotope tracing. RESULTS: 5-Lipoxygenase inhibitors nordihydroguaiaretic acid, AA861 and MK886, could suppress the expression of ICAM-1 mRNA in a375 was evaluated by Northern blot analysis. 1protein as well as of its mRNA in a375 cells and reduce the adhesion of a375 to EC 304. CONCLUSION: 5-Lipoxygenase inhibitors can inhibit the expression of ICAM-1 in human melanoma cells and may be valuable fortreatment of melanoma metastasis.
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