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以聚乙二醇PEG-6000为渗透剂模拟水分胁迫处理马铃薯试管苗,研究马铃薯试管苗在不同强度水分胁迫下的光合能力、渗透调节物质含量、膜脂过氧化和抗氧化特性等生理反应。结果表明:随着胁迫强度增大,马铃薯试管苗叶绿素、脯氨酸、丙二醛(MDA)与超氧化物歧化酶(SOD)活性发生了不同变化。抗旱性不同的马铃薯品种对水分胁迫的敏感程度也不同。其中水分胁迫后叶绿素含量低于对照;脯氨酸含量随着处理强度的增大显著增加,且以20%的PEG浓度处理增加最明显,抗旱性强的品种脯氨酸增幅较大;MDA含量随着胁迫强度的增大急剧增加,植物叶片的质膜透性上升,抗旱性强的品种增长幅度较小;PEG处理后SOD活性先上升后下降,抗旱性强的品种能维持较高的活性。
Potato plantlets were treated with polyethylene glycol PEG-6000 as penetrant to simulate water stress. The physiological responses of potato plantlets to photosynthesis, osmotic adjustment, membrane lipid peroxidation and antioxidation were studied under different water stress conditions. The results showed that the chlorophyll, proline, malondialdehyde (MDA) and superoxide dismutase (SOD) activity of potato plantlets varied with the increase of stress intensity. The susceptibility of potato varieties with different drought resistance to water stress is also different. Chlorophyll content was lower than that of the control under water stress; Proline content increased significantly with the increase of treatment intensity, and proline increased most obviously when treated with 20% PEG concentration; With the increase of the stress intensity, the membrane permeability of plant leaves increased sharply, and the growth of drought-resistant cultivars was less. The activity of SOD increased first and then decreased, while the drought-tolerant cultivars maintained high activity .