论文部分内容阅读
目的建立苍耳子中苍耳子噻嗪双酮苷的含量测定方法。方法采用乙醇提取、正丁醇萃取与硅胶柱色谱分离等方法制备苍耳子噻嗪双酮苷;采用HPLC法进行含量测定,色谱条件为:Dia-monsilTM ODS色谱柱(250 mm×4.6 mm,5μm),以质量分数为0.04%的磷酸溶液-乙腈(体积比为90∶10)为流动相,检测波长为254 nm,流速为1 mL.min-1。结果苍耳子噻嗪双酮苷质量浓度在1～100 mg.L-1内线性关系良好(r=0.999 9,n=6),平均回收率为100.2%,RSD为2.3%(n=9)。结论HPLC法适合测定苍耳子中苍耳子噻嗪双酮苷的含量。
Objective To establish a method for the determination of Xanthium Diazone in Fructus Xanthium. Methods Sesquiterpetazin was prepared by ethanol extraction, n-butanol extraction and silica gel column chromatography. The content of Saxazone was determined by HPLC. The chromatographic conditions were: Dia-monsilTM ODS column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of 0.04% phosphoric acid solution-acetonitrile (90:10 by volume) with a detection wavelength of 254 nm and a flow rate of 1 mL · min-1. Results The calibration curve was linear between 1 and 100 mg.L-1 (r = 0.999 9, n = 6), with an average recovery of 100.2% and RSD of 2.3% (n = 9 ). Conclusion HPLC method is suitable for the determination of Xanthium Dioscin in Fructus Xanthium.