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目的:探讨中药黄连素对子宫颈癌Hela细胞株的增殖抑制和诱导凋亡的作用。方法:以不同浓度的黄连素(10、20、40、80μmol/L),分12、24、48、72h四个时间点处理Hela细胞,光镜观察细胞形态变化;用四甲基偶氮唑蓝(MTT)法测定细胞增殖抑制率;用透射电子显微镜及琼脂糖凝胶电泳检测凋亡发生情况。结果:黄连素可抑制Hela细胞增殖,作用呈明显的时效和量效关系,差异有统计学意义(P<0.01);黄连素可诱导Hela细胞凋亡,透射电子显微镜下可见凋亡细胞;琼脂糖凝胶电泳出现细胞凋亡典型的DNA“梯状”条带。结论:黄连素体外对Hela细胞具有增殖抑制作用和促进凋亡作用。
Objective: To investigate the effect of berberine on proliferation and apoptosis of cervical cancer Hela cell line. Methods: Hela cells were treated with different concentrations of berberine (10, 20, 40 and 80μmol / L) for 12, 24, 48 and 72h. The morphological changes were observed with light microscope. Cell proliferation inhibition rate was measured by MTT method. Apoptosis was detected by transmission electron microscopy and agarose gel electrophoresis. Results: Berberine could inhibit the proliferation of Hela cells in a time-and dose-dependent manner. The difference was statistically significant (P <0.01). Berberine induced apoptosis of Hela cells and apoptotic cells were observed under transmission electron microscope. Typical DNA “ladder” bands appear in the apheresis gel electrophoresis. Conclusion: Berberine can inhibit proliferation and promote apoptosis of Hela cells in vitro.