论文部分内容阅读
河北杨(Populus hopeiensis)是我国华北和西北主要造林树种之一,比毛白杨耐瘠薄和干旱,但扦插难成活。我们试图用组织培养法解决河北杨难无性繁殖的问题。一、组培材料和方法选用两种材料,一种是用母树上的生长枝;一种是伐根萌出的幼枝。将枝条取下后剪成长5—6厘米(以顶端5—6厘米为好),放在70%的乙醇(半分钟)和10%的安替福民(5—10分钟)或0.1%的氯化汞(5—10分钟)中进行消毒,再在无菌水中洗刷三次,用消毒卫生纸吸干水分,然后在无菌条件下切成3—5毫米长的小段,置于培养基上。为了比较枝切段、叶柄切段及叶片小块的分化效果,把这三种材料分开培养。为筛选出适宜的培养基,我们采用的是正交法,以MS培养基为基础,附加NAA,
Populus hopeiensis is one of the main afforestation species in North and Northwest China. Compared with Populus tomentosa, Populus hopeiensis is resistant to infertility and drought, but cutting propagation is difficult to survive. We tried to use tissue culture method to solve the problem of clonal propagation in Hebei Province. First, the tissue culture materials and methods used two kinds of materials, one is growing branches on the mother tree; one is sprouting young branches. The branches are removed and cut to grow 5-6 cm (preferably 5-6 cm top), placed in 70% ethanol (half a minute) and 10% antifumint (5-10 minutes) or 0.1% Mercuric chloride (5-10 min), washed three times in sterile water, blotted dry with sterile tissue paper, and cut into 3-5 mm long sections under aseptic conditions and placed on culture medium. In order to compare the differentiation of shoots, petioles and leaves, the three materials were cultured separately. In order to filter out the appropriate medium, we used the orthogonal method, based on MS medium, add NAA,