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本文建立了一种新的ＩＦＮ－γ检测方法。此方法利用ＩＦＮ－γ能够诱导ＭΦ产生并释放ＮＯ的原理，用微量显色技术对其衍生物亚硝酸盐ＮＯ进行定量测定。结果表明，样品中ＩＦＮ－γ的含量与ＭΦ释放ＮＯ的量有很好的相关性，抑制试验证实这种方法具有特异性。与ＩＦＮ－γ一起加入少量ＬＰＳ可以提高方法的敏感性和特异性。其它细胞因子，如ＩＬ－１、ＩＬ－２、ＴＮＦ、ＩＦＮ－β，对ＮＯ释放量的影响较小，可以忽略不计。此方法可供检测实验动物的ＩＦＮ－γ之用，有较高实用价值。
This article established a new IFN-γ detection method. This method utilizes the principle that IFN-γ induces the production of MΦ and releases NO, and the trace nitrite NO is quantitatively determined by a trace amount chromogenic technique. The results showed that there was a good correlation between the amount of IFN-γ in the sample and the amount of NO released by MΦ, and the inhibition test confirmed the specificity of this method. Addition of a small amount of LPS together with IFN- [gamma] increases the sensitivity and specificity of the method. Other cytokines, such as IL-1, IL-2, TNF, IFN-β, had little effect on NO release and were negligible. This method can be used for the detection of experimental animal IFN-γ, with high practical value.