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背景:研究在运动应激状态下肠道屏障损伤发生机制及规律,将为运动应激肠道屏障保护剂的研制提供理论依据。目的:观察不同强度游泳运动后大鼠肠屏障的变化。设计:随机对照动物实验。单位:湖北大学运动人体科学实验室和武汉大学医学院基础实验室。材料:选取3周龄健康雄性SD大鼠36只,随机分为对照组10只、适度运动组12只和过度性运动组14只,3组大鼠饲养条件一致。方法:①对照组平时不运动。②适度运动组:进行无负重游泳,前3d适应性游30min,并在1周内逐渐延长至60min,然后每天游泳1次,每周6次,训练6周。③过度运动组:前3d适应性游30min,并在1周内延长时间至120min,训练1周后,进行过度游泳训练。然后每天游泳1次,每周6次,持续4周。最后2周,每天早、晚1次,每周6次。主要观察指标:①大鼠肠屏障参数:血浆内毒素,肠黏膜通透性,细菌移位率。②大鼠肠黏膜组织结构。结果:①大鼠肠屏障参数结果:大鼠过度运动后血浆内毒素增加2倍,肠黏膜通透性提高2.5倍,细菌移位率增加230%。②适度运动对大鼠肠黏膜组织结构无明显影响,过度运动使大鼠肠上皮细胞内高尔基复合体和粗面内质网扩张,上皮细胞高度水肿,炎细胞浸润。结论:适度运动可以改善机体肠功能;过度运动造成机体肠屏障损伤,导致消化系统出现病理性征候群。
BACKGROUND: To study the mechanism and regulation of intestinal barrier injury under exercise stress will provide a theoretical basis for the development of protective agent of gut barrier. Objective: To observe the changes of intestinal barrier in rats after swimming with different intensities. Design: Randomized controlled animal experiments. Unit: Hubei University Sports Human Science Laboratory and Wuhan University Medical College Basic Laboratory. MATERIALS: Thirty-six healthy male SD rats aged 3 weeks were randomly divided into control group (n = 10), moderate exercise group (n = 12) and excessive exercise group (n = 14). Methods: ① control group usually do not exercise. ② moderate exercise group: no-load swimming, the first 3d adaptive travel 30min, and gradually extended to 60min within 1 week, and then once a day swimming, 6 times a week, training for 6 weeks. ③ over-exercise group: the first three days of adaptive travel 30min, and in 1 week to extend the time to 120min, 1 week after training, excessive swimming training. Then swim once a day, 6 times a week for 4 weeks. The last two weeks, every morning and evening 1 times, 6 times a week. MAIN OUTCOME MEASURES: ① Rat intestinal barrier parameters: plasma endotoxin, intestinal mucosal permeability, bacterial translocation rate. ② rat intestinal mucosa tissue structure. Results: (1) Parameters of intestinal barrier in rats: The level of plasma endotoxin increased 2-fold, the intestinal mucosal permeability increased 2.5-fold and the bacterial translocation rate increased by 230% after over-exercising. (2) Moderate exercise had no significant effect on the intestinal mucosal structure in rats. Excessive exercise dilated the Golgi complex and the rough endoplasmic reticulum in the intestinal epithelial cells of rats, with highly edema of epithelial cells and inflammatory cell infiltration. Conclusion: Moderate exercise can improve the intestinal function of the body. Excessive exercise can damage the intestinal barrier of the body and lead to pathological signs in the digestive system.