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目的:同时作用于ASICs及GABA受体,探讨两者对肺泡液体的清除的影响。方法:先通过膜片钳技术,研究肺泡上皮ASICs和GABA受体在不同PH值下及GABA受体激活或抑制条件下的相互作用;70只雄性Wistar大鼠随机分成空白对照组(NS)、LPS组(LPS)、LPS+PBS组(PBS)、LPS+PcTX-1组(PcTX-1)、LPS+GABA组(GABA)、LPS+Bicuculline组(Bicuculline)、LPS+PBS+Bicuculline组(PBS+Bicuculline),以LPS制作大鼠肺水肿模型,以PBS、PcTX-1作用于ASICs;以GABA、Bicuculline作用于GABA受体,采用重量分析法计算肺组织含水量。结果:GABA受体可逆性抑制ASICs电流;激活ASICs抑制GABA受体门控电流。同时激活ASICs及抑制GABA受体不但可以减少肺组织含水量,并且减少量大于单独作用于两者之和。可以进一步推断,ASICs及GABA受体存在于肺泡表面,两者可能以某种形式形成蛋白质复合体,相互调节作用于肺水肿的发生,增加肺泡液体的清除。
Objective: Simultaneous effect on ASICs and GABA receptors, to explore both the impact of alveolar fluid clearance. Methods: The patch clamp technique was used to study the interaction of ASICs and GABA receptors on the alveolar epithelium under different PH values and GABA receptor activation or inhibition. Seventy male Wistar rats were randomly divided into blank control group (NS) LPS group, PBS, LPS + PcTX-1 group, LPS + GABA group, LPS + Bicuculline group, LPS + PBS + Bicuculline group + Bicuculline). The pulmonary edema model was induced by LPS, and ASICs were treated with PBS and PcTX-1. GABA and Bicuculline were administered to GABA receptor. Gravimetric method was used to calculate the lung water content. Results: GABA receptor reversibly inhibits ASICs currents; activating ASICs inhibits GABA receptor-gated currents. Simultaneous activation of ASICs and inhibition of GABA receptors not only reduced lung water content, but also decreased more than the sum of the two alone. It can be further deduced that ASICs and GABA receptors are present on the surface of alveoli, both of which may form protein complexes in some form, which regulate the occurrence of pulmonary edema and increase the clearance of alveolar fluid.