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本实验观察了正常及高血压大鼠红细胞膜Ｃａ２＋，Ｍｇ２＋－ＡＴＰ酶对不同浓度Ｃａ２＋及Ｍｇ２＋的反应。结果表明：（１）在自发性高血压大鼠（ＳＨＲ），此酶最适反应的Ｃａ２＋浓度为１０－６ｍｏｌ／Ｌ；ＷＫＹ大鼠为１０－４ｍｏｌ／Ｌ；两肾一环型肾性高血压大鼠（ＲＨＲ）为１０－７ｍｏｌ／Ｌ，Ｗｉｓｔａｒ大鼠为１０－４ｍｏｌ／Ｌ，Ｃａ２＋高于以上各相应浓度时该酶活性受到抑制；（２）作为该酶激动剂的Ｍｇ２＋有其最适激活浓度，在ＷＫＹ大鼠、ＲＨＲ及Ｗｉｓｔａｒ大鼠均为４．５ｍｏｌ／Ｌ；（３）高浓度Ｍｇ２＋（３６．０ｍｏｌ／Ｌ）可以逆转高浓度Ｃａ２＋对该酶的抑制作用。以上结果表明，底物Ｃａ２＋浓度的变化对Ｃａ２＋，Ｍｇ２＋－ＡＴＰ酶的催化活性影响极大，该酶活性的促发及维持必须有适宜浓度的Ｍｇ２＋。高血压时此酶对Ｃａ２＋的敏感性增高，且Ｍｇ２＋对酶保护作用更为明显。本结果提示，胞内高浓度Ｃａ２＋不仅是高血压发生的原因之一，并可能与其发展及恶化有关。
This experiment observed the normal and hypertensive rat erythrocyte membrane Ca2 +, Mg2 + -ATP enzyme on different concentrations of Ca2 + and Mg2 + response. The results showed that: (1) In spontaneously hypertensive rats (SHR), the optimum concentration of Ca2 + for this enzyme was 10-6 mol / L; for WKY rats 10-4 mol / L; The blood pressure (RHR) of 10-7mol / L, Wistar rats was 10-4mol / L, Ca2 + higher than the corresponding concentration of the above enzyme activity was inhibited; (2) as the enzyme agonist Mg2 + The optimal concentration was 4.5 mol / L in WKY rats, RHR and Wistar rats. (3) High concentrations of Mg2 + (36.0 mol / L) could reverse the inhibitory effect of high concentrations of Ca2 + on this enzyme. The above results show that the change of substrate Ca2 + concentration has a great influence on the catalytic activity of Ca2 + and Mg2 + -ATPase. The promotion and maintenance of this enzyme activity must have appropriate concentration of Mg2 +. This enzyme increased the sensitivity of Ca2 + to hypertension, and the protective effect of Mg2 + on the enzyme was more obvious. The results suggest that intracellular high concentration of Ca2 + is not only one of the causes of hypertension, and may be related to its development and deterioration.