利用高粱根作为实验材料 ,用不连续蔗糖密度梯度和DextranT - 5 0 0 /PEG - 335 0两相法探讨了纯化质膜的过程 ,以及去垢剂对K+运输蛋白的增溶效率。结果表明 ,由 6mL 4 0 % (W /W ) ,4mL 34% (W /W )和 4mL 2 2 % (W /W )蔗糖组成的不连续蔗糖梯度 ,经 80 0 0 0×g离心 3h后 ,在 34%～ 4 0 %界面处富含质膜。在质膜K+运输蛋白的增溶效果上 ,6 5mmol/LCHAPS最为理想 Sorghum root was used as the experimental material, and the process of purification of plasma membrane and the solubilization efficiency of detergent to K + transport protein were investigated by discontinuous sucrose density gradient and DextranT - 500 / PEG - 335 0 two - phase method. The results showed that a discontinuous sucrose gradient composed of 6 mL 40% (w / w), 4 mL 34% (w / w) and 4 mL 2 2% (w / w) sucrose was centrifuged at 80,000 g for 3 h , Rich in plasma membrane at 34% ~ 40% interface. In the plasma membrane K + transport protein solubilization effect, 6 5mmol / LCHAPS the most ideal