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本实验以乙脑病毒京卫研1株腹腔接种小鼠,应用PAP法对常规石蜡包埋的小鼠脑组织中乙脑病毒抗原的分布等进行了观察;描述了病毒抗原阳性细胞出现的时间、部位、分布特点和种类以及病毒抗原在阳性细胞内的分布类型和变化规律,并对其中的一些观察结果与HE染色切片和电镜观察结果进行了对比和讨论;发现除其它神经细胞外,小脑皮质的颗粒细胞、高尔基细胞、分子层以及小脑内核的神经细胞中也可检出病毒抗原阳性细胞;进一步证实,感染后期,部分感染细胞的胞浆和核内可出现病毒抗原,认为主要是由于病毒粒子从胞浆进入核内所致,并发现此类型阳性细胞为坏死细胞;另外讨论了有关PAP法染色的几个问题。
In this experiment, mice were inoculated intraperitoneally with JEV 1, and the distribution of JE virus antigens in the brain tissue of conventional paraffin-embedded mice was observed by PAP method. The time when virus antigen positive cells appeared , The distribution characteristics and types of the virus, the type and variation of the distribution of virus antigens in positive cells, and some of the observations were compared with the HE staining sections and electron microscopy observations and found that in addition to other neurons, the cerebellum Cortical granulosa cells, Golgi cells, the molecular layer and cerebellar nuclei in the nerve cells can also be detected in the positive antigen-positive cells; further confirmed that late infection, some infected cells in the cytoplasm and nuclear antigen can be considered, mainly due to Virus particles from the cytoplasm into the nucleus due to this type of positive cells found to be necrotic cells; In addition to discuss the PAP staining of several issues.