应用基因组原位杂交及RFLP标记鉴定小麦中的大麦染色体

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用生物素(Biotin-6-dUTP)标记的大麦Betzes基因组DNA作探针,以普通小麦中国春总DNA作封阻进行基因组原位杂交(Genomeinsituhybridization,简称GISH),从13株小麦-大麦杂交后代中鉴定出2个含有3条大麦Betzes2H染色体的材料(2n=43);2个2H单体异代换系(2n=42);7个2H二体异代换系(2n=42)。用已定位在小麦第2部分同源群短臂上的探针psr131进行RFLP分析,结果表明大麦Betzes、代换系A5有1条区别于小麦中国春的特异带,A5的2条2A染色体被大麦Betzes的2条2H染色体所代换。GISH及RFLP的准确鉴定及小麦柎舐2H二体异代换系的首次获得,为向小麦导入2H染色体上的有用基因提供了宝贵材料。
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