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目的:应用系统生物学方法模拟分析丹红注射液中丹参与红花配伍的分子机制。方法:采用TCMGene DIT和Agilent literature search(ALS)结合搜索方式,挖掘丹参、红花各自作用的蛋白,在BIND,Bio GRID等数据库中查询蛋白间关联,分别构建丹参、红花及丹红注射液蛋白相互作用网络,应用Merge程序中的difference和intersection功能比较网络间异同。结果:采用intersection分析得丹参和红花共有的高连接区蛋白网络含934个蛋白,经cluster ONE方法提取出P<0.05的高连接区蛋白子网络4个,Bi NGO插件的基因本位论聚类分析表明,其主要与RNA代谢,核因子kappa B(nuclear factor kappa B,NF-κB)级联反应,脂质代谢,Rho蛋白及小GTP酶调节4类生物学途径相关。将丹红注射液蛋白相互作用网络与丹参、红花共有高连接区蛋白网络进行difference分析,得由1 431个蛋白构成的差异网络,其主要影响细胞增殖、迁移和自噬等。结论:本研究采用系统生物学方法模拟丹参和红花的配伍机制,其可能主要在RNA代谢,NF-κB级联反应以及细胞增殖、迁移和自噬等生物学途径上协同发挥防治疾病的作用。
OBJECTIVE: To study the molecular mechanism of the compatibility of Salvia miltiorrhiza with Safflower in Danhong injection by systematic biology method. Methods: Using the combination of TCMGene DIT and Agilent literature search (ALS), the proteins of Salvia miltiorrhiza and Safflower were screened, and the protein associations were searched in BIND and Bio GRID databases to construct Salvia miltiorrhiza, Safflower and Danhong injection respectively The protein interaction network uses the difference and intersection functions in the Merge program to compare similarities and differences between networks. Results: Using intersection analysis, 934 proteins were found in the common high-junction protein network of Salvia miltiorrhiza and safflower, 4 of the high-protein sub-networks with P <0.05 were extracted by cluster ONE method, The results showed that it mainly related to four biological pathways, such as RNA metabolism, nuclear factor kappa B (NF-κB) cascade, lipid metabolism, Rho protein and small GTPase regulation. Difference network between Danhong injection protein interaction network and Salvia miltiorrhiza and safflower shared a high junction protein network, which resulted in a difference network consisting of 1 431 proteins, which mainly affected cell proliferation, migration and autophagy. Conclusion: In this study, we used the method of systematic biology to simulate the compatibility mechanism between Salvia miltiorrhiza and Safflower. It may play a role in disease control in the biological pathway of RNA metabolism, NF-κB cascade, cell proliferation, migration and autophagy .