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目的研究注射用脑蛋白水解物(Ⅰ)对血管性痴呆大鼠的保护作用。方法采用改良两血管阻断法制备血管性痴呆(VaD)大鼠模型,假手术组分离颈总动脉后缝合切口。模型大鼠随机分为模型组,脑蛋白水解物注射液(Cerebrolysin,阳性药,10 mg/kg)组,注射用脑蛋白水解物(Ⅰ)高、中、低剂量(20、10、5 mg/kg)组,尾iv给药,每天1次,连续给药2周;假手术组给予等体积生理盐水。采用Y迷宫和避暗实验法评价大鼠学习记忆能力;ELISA法检测VaD大鼠血清中胰岛素样生长因子1(IGF-1)水平。结果与模型组比较,注射用脑蛋白水解物(Ⅰ)显著增加VaD大鼠在Y迷宫实验的正确次数,显著降低在避暗实验的潜伏期时间,并显著提高VaD大鼠血清IGF-1水平;上述作用与同剂量Cerebrolysin比较无显著差异。结论注射用脑蛋白水解物(Ⅰ)能提高VaD大鼠学习记忆能力,其机制可能与提高机体IGF-1水平,促进脑损伤后的神经元修复和生长有关。
Objective To study the protective effect of injectable brain protein hydrolyzate (Ⅰ) on vascular dementia rats. Methods Vascular Dementia (VaD) rat model was prepared by modified two-vessel occlusion method. The sham operation group was sutured from the common carotid artery. The model rats were randomly divided into model group, Cerebrolysin (positive drug, 10 mg / kg) group, high, medium and low dose of brain protein hydrolyzate for injection / kg) group, tail iv administration, once a day, continuous administration for 2 weeks; sham operation group was given equal volume of normal saline. The learning and memory abilities of rats were evaluated by Y-maze and dark-avoidance test. The serum insulin-like growth factor 1 (IGF-1) levels were measured by ELISA. Results Compared with the model group, the brain protein hydrolyzate for injection (Ⅰ) significantly increased the correct number of VaD rats in the Y maze test, significantly reduced the incubation time in avoiding darkness and significantly increased the serum IGF-1 level in VaD rats. The above effects and the same dose Cerebrolysin no significant difference. Conclusion Injection of brain protein hydrolyzate (Ⅰ) can improve the learning and memory abilities of VaD rats. The mechanism may be related to the increase of IGF-1 level and the promotion of neuronal repair and growth after brain injury.