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目的:探讨丹参酮ⅡA对HeLa宫颈癌细胞凋亡的影响及其作用机制。方法:应用MTT比色法检测不同浓度丹参酮ⅡA对HeLa宫颈癌细胞的增殖抑制,采用烟酸己可碱33258染色法观察丹参酮ⅡA对HeLa细胞凋亡的影响,并用半定量逆转录聚合酶链反应法检测用药后Bcl-2和bax基因mRNA的表达水平。结果:丹参酮ⅡA对HeLa细胞增殖的抑制作用呈剂量依赖性;它能促使HeLa细胞发生凋亡;用药48h后Bcl-2基因mRNA的表达水平明显降低,而bax基因的表达则无明显变化。结论:丹参酮ⅡA对HeLa细胞的增殖具有显著的抑制及诱导凋亡作用。
Objective: To investigate the effect of tanshinone II A on HeLa cervical cancer cell apoptosis and its mechanism of action. METHODS: MTT colorimetric assay was used to detect the proliferation inhibition of HeLa cervical cancer cells with different concentrations of tanshinone IIA. The effect of tanshinone II A on the apoptosis of HeLa cells was observed by Hoechst 33258 staining and semi-quantitative reverse transcriptase polymerase chain reaction was used. The expression levels of Bcl-2 and bax mRNA were measured after drug treatment. RESULTS: The inhibitory effect of tanshinone II A on proliferation of HeLa cells was dose-dependent; it could induce apoptosis of HeLa cells; the expression of Bcl-2 mRNA decreased significantly after 48 hours of treatment, but there was no significant change in the expression of bax gene. Conclusion: Tanshinone IIA can significantly inhibit the proliferation of HeLa cells and induce apoptosis.