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目的 研究大鼠胚胎神经干细胞移植治疗脑出血的可行性。方法 从孕龄 16天的大鼠胚胎脑组织中分离、培养神经干细胞并诱导其分化 ,通过免疫组化学技术研究其特性。制作大鼠脑出血模型 ,3天后将未分化的神经干细胞注入血肿同侧或对侧的尾状核内。记录损伤和移植后的大鼠运动功能。不同时间杀死大鼠 ,研究移植后的干细胞在体内分化和迁徙的情况。结果 实验中分离、培养的神经干细胞体外能够被诱导分化成神经元、少突胶质细胞和星形胶质细胞。血肿同侧移植干细胞的大鼠运动功能的改善显著好于血肿对侧移植干细胞组及未移植干细胞的对照组。免疫组化方法证实移植后的干细胞在体内可分化成神经元和胶质细胞 ,并向损伤区域迁徙。结论 大鼠胚胎神经干细胞体内、体外均具有多向分化潜能 ,其分化成各种类型神经细胞的比例与所处的外界环境有关。在脑内靠近损伤部位移植胚胎神经干细胞后能够有效改善脑出血动物的运动功能。
Objective To investigate the feasibility of rat embryonic neural stem cell transplantation for cerebral hemorrhage. Methods Neural stem cells (NSCs) were isolated and cultured from the embryonic brain tissue of 16-day gestational age rats to induce their differentiation. Their characteristics were studied by immunohistochemistry. Rat models of intracerebral hemorrhage were made and, three days later, undifferentiated neural stem cells were injected into the caudate nucleus ipsilateral or contralateral to the hematoma. Injury and posttransplantation rat motor function was recorded. Rats were killed at different times to investigate the differentiation and migration of stem cells after transplantation in vivo. Results The neural stem cells isolated and cultured in vitro could be induced to differentiate into neurons, oligodendrocytes and astrocytes in vitro. The improvement of motor function in ipsilateral stem cells of hematoma was significantly better than that of contralateral hematopoietic stem cell transplantation group and control group of non-transplanted stem cells. Immunohistochemistry confirmed that the stem cells after transplantation could differentiate into neurons and glial cells in vivo and migrated to the damaged area. Conclusion The neural stem cells of rat embryos have multi-directional differentiation potential both in vitro and in vivo. The percentage of neural stem cells differentiated into various types of neural cells is related to the external environment. Transplantation of embryonic neural stem cells in the brain near the injury site can effectively improve the motor function of animals with intracerebral hemorrhage.