目的研究甲醛吸入性急性肺水肿模型大鼠心、肺、脑、肝和血清蛋白浓度及一氧化氮合酶(NOS)活力的变化,为研究此类肺水肿提供实验依据。方法健康成年清洁级SD大鼠18只随机分为对照组和模型组,在染毒箱内以“静态+喷雾”吸入40%甲醛2h复制急性肺水肿模型,8h后眼球采血并处死,按试剂盒说明书测定血清及心、肺、脑、肝组织匀浆的蛋白浓度和NOS活力,并计算心肺系数、心指数和肺指数,肺组织作常规病理检查。对照组将甲醛换为生理盐水,处理方法同模型组。结果与生理盐水对照组比较,模型组心肺系数显著增大(P<0.05),病理切片显示模型组出现肺组织间隙增大、大量有核细胞浸润等肺水肿改变;血清、心脏、脑和肺匀浆蛋白浓度显著增加(P<0.05或P<0.01),肝蛋白浓度差异无统计学意义(P>0.05);血清、心、肺、脑、肝NOS活力无统计学意义(均P>0.05)。结论甲醛吸入性急性肺水肿大鼠血清、心脏、脑和肺蛋白含量增加,NOS活力变化不大。 Objective To study the changes of protein concentration and nitric oxide synthase (NOS) activity in heart, lung, brain, liver and serum of rats with inhalation of acute pulmonary edema induced by formaldehyde and provide experimental evidence for the study of such pulmonary edema. METHODS: Eighteen healthy adult SD rats were randomly divided into control group and model group. Acute pulmonary edema model was induced by inhalation of 40% formaldehyde in “static + spray” The protein concentration and NOS activity of serum and heart, lung, brain and liver homogenates were measured according to kit instructions and cardiorespiratory index, cardiac index and lung index were calculated, and the lung tissues were routinely examined for pathology. For the control group, formaldehyde was changed into normal saline, and the treatment method was the same as that of the model group. Results Compared with the saline control group, the cardio-pulmonary index of the model group was significantly increased (P <0.05). Pathological examination showed that pulmonary interstitial space increased and a large number of nucleated cells infiltrated pulmonary edema. The serum, heart, brain and lung (P <0.05 or P <0.01), but there was no significant difference in hepatic protein concentration (P> 0.05). The activity of NOS in serum, heart, lung, brain and liver had no statistical significance ). Conclusions The levels of serum, heart, brain and lung protein in rats with formaldehyde-induced acute pulmonary edema increase but the activity of NOS changes little.