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用家兔及人和家兔外周血淋巴细胞为对象,分别对热耐受及非耐受动物组织和细胞的热应激蛋白(HSP)从分子量和等电点两个方面进行了分析比较,对HSP合成动力学进行了观察,并对扫描电镜、透射电镜观察到的细胞形态学变化和细胞内HSP水平的联系作了分析。实验结果表明高温可使热耐受组和非热耐受组动物组织和细胞迅速合成一系列HSP。新合成的HSP种类在各观察对象中基本相同,以HSP70(68~74)kd及HSP85(80~90)kd及PI为6左右的多肽合成增加最为明显,同步伴随着一些小分子多肽(分子量40kd,PI8左右)合成减少。各实验组不同组织及细胞合成HSP间存在一定的差异。淋巴细胞中HSP合成动力学表明,受热后立即出现以HSP70为主的多肽合成显著增加,3h~6h后合成达到顶点,再逐渐降低至9h基本恢复至正常水平。淋巴细胞形态学观察发现,41℃受热30min可引起细胞大量合成HSP,而不造成细胞形态学改变;37℃6h后胞内HSP水平达到最大时,再在45℃受热15min,其形态学改变要比单纯45℃受热的细胞轻得多,且损伤多是可逆的。本研究认为高温使机体组织细胞产生HSP,它在热耐受形成而保护机体免受热损伤的机制中
Rabbits and human and rabbit peripheral blood lymphocytes were used as targets to analyze the heat stress protein (HSP) of heat tolerance and non-tolerance animal tissues and cells respectively from molecular weight and isoelectric point. The kinetics of HSP synthesis was observed, and the relationship between the morphological changes observed by SEM and TEM and the level of intracellular HSP was analyzed. The experimental results show that high temperature can make heat-tolerant group and non-heat-tolerant group of animal tissues and cells rapidly synthesize a series of HSP. The newly synthesized HSP species were basically the same in all the observed subjects. The most obvious increase was in the synthesis of peptides with HSP70 (68-74) kd, HSP85 (80-90) kd and PI 6, accompanied by some small molecule peptides (molecular weight 40kd, PI8 or so) decreased synthesis. The experimental groups of different tissues and cell synthesis of HSP there are some differences. The kinetics of HSP synthesis in lymphocytes showed that the HSP70-based peptide synthesis increased immediately after heating and reached the peak at 3h-6h, then gradually decreased to 9h and returned to normal. Lymphocyte morphological observation found that 41 ℃ heat 30min can cause a large number of cell synthesis of HSP, without causing morphological changes; 37 ℃ 6h after intracellular HSP levels reached the maximum, then at 45 ℃ for 15min, the morphological changes It is much lighter than the cells heated at 45 ℃, and the damage is mostly reversible. This study suggests that high temperature to make tissue cells produce HSP, it is in the formation of heat tolerance and protect the body from heat damage mechanism