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目的研究大鼠延髓内脏带(MVZ)内小胶质细胞和神经元对福尔马林诱发胃伤害性刺激的可塑性反应及其相互关系。方法经细塑料管向胃内注入2.5%福尔马林2ml诱发胃伤害性刺激,动物存活0.5h、1h、2h和3h处死,延髓切片进行抗Fos蛋白(标记神经元)或抗OX42(标记小胶质细胞)单一或双重标记的ABC法染色。结果1.动物清醒后,在5～10min内出现躁动不安,持续1～2h;2.胃黏膜有局灶性出血和脱落;3.MVZ内观察到Fos阳性神经元和OX42阳性小胶质细胞,两者分布相似,显示明显的定位特点;4.双标记显示MVZ内Fos阳性神经元周围有密集的OX42阳性细胞;5.OX42阳性小胶质细胞的反应先逐渐升高(0.5～2h),后(3h)又降低,Fos阳性神经元则呈逐渐升高的趋势。结论MVZ的小胶质细胞可能与神经元一起参与内脏痛的调节。
Objective To investigate the plasticity of the microglial cells and neurons in the medullary visceral zone (MVZ) of rats induced by formalin-induced gastric noxious stimulation and their correlation. Methods Stomach irritation was induced by injecting 2ml of 2.5% formalin into the stomach through a fine plastic tube. The animals were sacrificed at 0.5h, 1h, 2h and 3h after sacrifice. The anti-Fos protein (labeled neurons) or anti-OX42 Microglia) Single or double-labeled ABC staining. Results 1. After awake, motility was disturbed within 5 ~ 10 min and continued for 1 ~ 2 h. 2. There was focal bleeding and shedding of gastric mucosa. 3. Fos positive neurons and OX42 positive microglial cells were observed in MVZ , The two were similar in distribution and showed obvious positioning characteristics.4. Double labeling showed dense OX42 positive cells around Fos positive neurons in MVZ.5. The response of OX42 positive microglial cells was gradually increased (0.5-2h) , After (3h) and then decreased, Fos positive neurons showed a gradual upward trend. Conclusion MVZ microglia may participate in the regulation of visceral pain together with neurons.