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关于小鼠骨髓细胞染色体的制备方法早有报道,近年来多数研究者采用先给小鼠腹腔注射一定量的秋水仙素溶液,给药后2～4h活杀动物,取骨髓细胞制备染色体标本。但在实际工作中,按上述一般方法操作只适合于一般的核型分析研究,往往难以获得足够的中期染色体,不适用于科研中大量动物的骨髓细胞染色体样本分析之用,而且所需时间长(平均5～6h)。本文对小鼠骨髓细胞染色体标本制备方法进行了改进并结合有关问题报道如下。
The preparation of chromosomes in mouse bone marrow cells has been reported for a long time. In recent years, most researchers use the first injection of a certain amount of colchicine solution into the abdominal cavity of the mice, and kill the animals 2 to 4 hours after administration, and take bone marrow cells to prepare chromosome samples. However, in practical work, the general method described above is only suitable for general karyotype analysis studies. It is often difficult to obtain enough metaphase chromosomes, which is not suitable for the analysis of bone marrow cell chromosome samples of a large number of animals in scientific research, and takes a long time. (average 5-6h). This article has improved the preparation method of chromosome samples of mouse bone marrow cells and combined related issues as follows.