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应用核团微穿刺取样和放射免疫测定技术，观察了在吗啡依赖和戒断期间大鼠中枢催产素（ＯＴ）和精氨酸升压素（ＡＶＰ）含量的变化．结果表明，慢性吗啡处理（剂量由５，１０，１５，２０，３０，４０，５０ｍｇ·ｋｇ－１逐日递增，同一剂量ｉｐ每日３次）大鼠的视上核和伏隔核ＯＴ含量显著降低；蓝斑ＯＴ含量显著增加．纳洛酮（１０ｍｇ·ｋｇ－１，ｉｐ）可使正常大鼠视上核，腹侧被盖区，隔区和伏隔核的ＯＴ含量明显降低，而在吗啡依赖大鼠使视上核内ＯＴ含量增加，同时室旁核的ＯＴ含量也明显增加，而蓝斑的ＯＴ含量则明显降低．吗啡，纳洛酮及吗啡加纳洛酮处理均不明显影响各核团内ＡＶＰ的含量．以上结果表明：慢性吗啡处理可抑制大鼠视上核细胞合成ＯＴ，中枢其他部位ＯＴ的减少可能与视上核ＯＴ合成减少有关；纳洛酮可以部分拮抗吗啡对ＯＴ神经元的抑制作用．
The nucleus micro-puncture sampling and radioimmunoassay were used to observe the change of central oxytocin (OT) and arginine vasopressin (AVP) levels during morphine dependence and withdrawal. The results showed that the contents of OT in the supraoptic nucleus and nucleus accumbens in rats treated with chronic morphine (dosages increased day by day with the doses of 5, 10, 15, 20, 30, 40 and 50 mg · kg-1, and the same dose of ip three times daily) Reduce; blue spot OT content increased significantly. Naloxone (10 mg · kg-1, ip) significantly decreased the content of OT in the supraoptic nucleus, ventral tegmental area, septal area and nucleus accumbens in normal rats. However, in morphine-dependent rats, OT content increased, while paraventricular nucleus OT content also increased significantly, while the blue spot OT content was significantly reduced. Morphine, naloxone and morphine plus ganadalone did not significantly affect the content of AVP in each nucleus. The above results show that chronic morphine treatment can inhibit the synthesis of OT in the supraoptic nucleus of rats, and the reduction of OT in other parts of the central nervous system may be related to the decrease of OT synthesis in the supraoptic nucleus. Naloxone partially antagonizes the inhibitory effect of morphine on OT neurons.