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目的:探讨二精丸总多糖抗长期应激大鼠学习记忆障碍的作用机制。方法:采用长期激怒应激法造模,二精丸总多糖灌胃,给药结束后用Morris水迷宫法测定各组实验大鼠的学习记忆能力;采用酶联免疫法测定大鼠血清雌二醇(E2)、睾酮(T)、皮质酮(CORT)、E2/T值以及海马部位乙酰胆碱酯酶(AchE)含量。结果:二精丸总多糖组与模型组相比,平均潜伏期明显缩短(P<0.05),搜索方式明显改变,游泳路径显示在中环区和中心区百分比增加(P<0.05),平台区穿越次数增多(P<0.05);E2和E2/T值明显降低(P<0.05),T和CORT明显升高(P<0.05);海马部位AchE浓度明显减少(P<0.05)。结论:二精丸总多糖能提高长期激怒应激法所致肾阴虚模型大鼠学习记忆能力,同时降低E2和E2/T浓度;升高T和CORT浓度;抑制AchE活性。
OBJECTIVE: To investigate the mechanism of the effect of Erzhi Pill on the learning and memory impairment of long-term stress rats. Methods: Using long-term irritative stress model, the total polysaccharides of Erjing Pills were intragastrically administered. Morris water maze was used to measure the learning and memory abilities of the rats in each group. The serum of rats was determined by enzyme-linked immunosorbent assay. Alcohol (E2), testosterone (T), corticosterone (CORT), E2/T values and acetylcholinesterase (AchE) content in the hippocampus. RESULTS: Compared with the model group, the total polysaccharide of Erjing Pills was significantly shorter (P<0.05), the search pattern was significantly changed, swimming path showed a percentage increase in the Central District and the central area (P<0.05), and the number of crossings in the platform area was increased. Increased (P <0.05); E2 and E2/T values decreased significantly (P <0.05), T and CORT increased significantly (P <0.05); hippocampal AchE concentration decreased significantly (P <0.05). Conclusion: The total polysaccharides of Erjing Pill can improve the learning and memory ability of rats with kidney yin deficiency model induced by long-term anger stress, reduce the concentrations of E2 and E2/T, increase the concentration of T and CORT, and inhibit the activity of AchE.